Human G1/S-specific Cyclin-D1 ELISA Kit

Code CSB-E08286h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P24385
Lead Time 3-5 working days
Abbreviation CCND1
Protein Biological Process 1 Cell Cycle
Target Name cyclin D1
Alias BCL1, D11S287E, PRAD1, U21B31, B-cell CLL/lymphoma 1|G1/S-specific cyclin D1
Species Homo sapiens (Human)
Protein Biological Process 3 Cell cycle
Sample Types serum, plasma, cell culture supernates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
HGNC 1582
RGD 68384
MGI 88313
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Cyclin-D1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %86-92
1:2Average %105
Range %103-107
1:4Average %91
Range %82-94
1:8Average %95
Range %88-99
Recovery
The recovery of human Cyclin-D1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)8782-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.558 2.447 2.503 2.379
101.483 1.524 1.504 1.380
50.868 0.848 0.858 0.734
2.50.521 0.501 0.511 0.387
1.250.334 0.327 0.331 0.207
0.6250.267 0.256 0.262 0.138
0.3120.194 0.197 0.196 0.072
00.125 0.123 0.124
References
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Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Involvement in disease Multiple myeloma (MM)
Subcellular Location Nucleus, Cytoplasm, Membrane
Protein Families Cyclin family, Cyclin D subfamily
Database Links

HGNC: 1582

OMIM: 168461

KEGG: hsa:595

STRING: 9606.ENSP00000227507

UniGene: Hs.523852

Pathway Hedgehog signaling pathway
Hippo signaling pathway
Jak-STAT signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Wnt signaling pathway
Focal adhesion
Cell cycle
Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
Apelin signaling pathway
FoxO signaling pathway

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