Human Galectin-2(LGALS2) ELISA kit

Instructions
Code CSB-EL012886HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lectin, galactoside-binding, soluble, 2
Alternative Names Beta galactoside binding lectin L14 II ELISA Kit; Beta-galactoside-binding lectin L-14-II ELISA Kit; CBP35 ELISA Kit; Gal-2 ELISA Kit; GAL3 ELISA Kit; GALBP ELISA Kit; Galectin 2 ELISA Kit; Galectin-2 ELISA Kit; GALIG ELISA Kit; HL14 ELISA Kit; Lactose binding lectin 2 ELISA Kit; Lactose-binding lectin 2 ELISA Kit; LEG2_HUMAN ELISA Kit; LGALS2 ELISA Kit; MAC2 ELISA Kit; S Lac lectin 2 ELISA Kit; S-Lac lectin 2 ELISA Kit
Abbreviation LGALS2
Uniprot No. P05162
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.312 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human galectin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 91
Range % 87-98
1:10 Average % 102
Range % 99-106
1:20 Average % 93
Range % 87-98
1:40 Average % 92
Range % 88-96
Recovery
The recovery of human galectin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 92-103
EDTA plasma (n=4) 93 89-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
80 2.488 2.514 2.501 2.325
40 2.109 2.233 2.171 1.995
20 1.656 1.622 1.639 1.463
10 0.918 0.955 0.937 0.761
5 0.537 0.536 0.537 0.361
2.5 0.359 0.366 0.363 0.187
1.25 0.266 0.277 0.272 0.096
0 0.172 0.180 0.176  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function This protein binds beta-galactoside. Its physiological function is not yet known.
Gene References into Functions
  1. Study observed decreased methylation at the DHCR24 locus in offspring of women with active pregnancy eating disorders (ED) and increased methylation at the LGALS2 locus in offspring of women with past ED compared to controls. PMID: 29093763
  2. Data show that lactose binding to galectin-2 (Gal-2) stabilizes the lectin's conformation. PMID: 27563008
  3. Gal-3 was significantly downregulated only in the extravillous trophoblast of intrauterine growth restriction ( IUGR) placentas. In contrast, expressions of gal-2 and gal-13 were downregulated in both villous and extravillous trophoblast cells of IUGR placentas. PMID: 27070577
  4. Galectin-2 binding to different circulating human monocyte subsets depends on monocyte surface expression levels of CD14. It skews human macrophages to a M1-like proinflammatory phenotype. PMID: 25884209
  5. PPARG rs1152002, AGTR1 rs5186, CXCL16 rs3744700 and LGALS2 rs7291467 polymorphisms may be closely related to the development of coronary heart disease PMID: 26045830
  6. Lower LGALS2 gene expression is associated with acute myeloid leukemia. PMID: 25953264
  7. Data suggest that expression of galectin 2 and galectin 2 mRNA is down-regulated in placental extravillous trophoblast and decidua in women with pre-eclampsia as compared to women with normal term pregnancy. PMID: 25707742
  8. The increased circulation of galectins -2, -4 and -8 in cancer patients contributes substantially to the increased circulation of G-CSF, IL-6 and MCP-1 by interaction with the blood vascular endothelium PMID: 24384681
  9. Cys57Met (single-site) mutant and its monoPEGylated derivative can markedly reduce binding of galectin-2 to physiological binding sites PMID: 23581621
  10. results identify galectin-2 as a novel inhibitor of arteriogenesis and its modulation may constitute a new therapeutic strategy for the stimulation of arteriogenesis in patients with coronary artery disease. PMID: 21831908
  11. LTA and LGALS2 polymorphisms affect the subclinical phenotype of the coronary artery, which predisposes to the incidence of myocardial infarction. PMID: 22310064
  12. low expression of galectin-2 was significantly associated with lymph node metastasis and advanced clinical stage in patients with gastric cancer PMID: 22015694
  13. These results suggested differences in the inflammatory response to malaria in children and adults with polymorphisms of the galectin-2 gene. PMID: 20500087
  14. the LTA gene and LGALS2-C3279T are not associated with coronary artery disease PMID: 19726041
  15. Our case-control association study in a Japanese population showed that a single nucleotide polymorphism in LGALS2 encoding galectin-2 is significantly associated with susceptibility to myocardial infarction PMID: 15129282
  16. This study classifies galectin-2 as proapoptotic effector for activated T cells, raising a therapeutic perspective. PMID: 15356130
  17. Fasting plasma glucose and serum insulin were statistically significantly associated with LGALS2 PMID: 16468038
  18. Galectin 2 induces surface phosphatidylserine exposure in a carbohydrate-dependent fashion in activated, but not resting, human neutrophils and in several leukocyte cell lines. PMID: 16940423
  19. no significant association of allele frequency with risk of myocardial infarction PMID: 17098239
  20. polymorphism of LGALS2 was not associated with the severity of coronary atherosclerosis in Japanese and Korean populations PMID: 17493152
  21. Data show that galectin-2 SNPs are not associated with myocardial infarction in two different German populations. PMID: 17497114
  22. Galectin-2 was present in nuclei of Geneti-cally engineered human colon carcinoma cells with stable ectopic expression. PMID: 17999373
  23. Data show that genotypes for the 3279C-->T polymorphism (rs7291467) of LGALS2 was associated (P<0.05) with the prevalence of atherothrombotic cerebral infarction. PMID: 18506375
  24. Galectin-2 (LGALS2) 3279C/T polymorphism may be independently associated with diastolic blood pressure in patients with rheumatoid arthritis. PMID: 19330599
  25. Galectin-2 is strongly expressed in inflammatory skin of autoimmune-prone transgenic mice, indicating that galectin-2 is up-regulated upon experimental cutaneous inflammation. PMID: 19380789

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Database Links

HGNC: 6562

OMIM: 150571

KEGG: hsa:3957

STRING: 9606.ENSP00000215886

UniGene: Hs.531776

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