Human Hepatocyte growth factor-regulated tyrosine kinase substrate(HGS) ELISA kit

Instructions
Code CSB-EL010329HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name hepatocyte growth factor-regulated tyrosine kinase substrate
Alternative Names HRS, Vps27, ZFYVE8, human growth factor-regulated tyrosine kinase substrate
Abbreviation HGS
Uniprot No. O14964
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HGS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 88
Range % 84-93
1:4 Average % 92
Range % 88-96
1:8 Average % 98
Range % 92-103
Recovery
The recovery of human HGS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-100
EDTA plasma (n=4) 105 98-113
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.619 2.493 2.556 2.460
750 1.657 1.742 1.700 1.604
375 0.989 0.976 0.983 0.887
187.5 0.527 0.509 0.518 0.422
93.75 0.284 0.291 0.288 0.192
46.88 0.229 0.220 0.225 0.129
23.44 0.151 0.148 0.150 0.054
0 0.094 0.097 0.096  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Subcellular Location Cytoplasm, Early endosome membrane, Peripheral membrane protein, Cytoplasmic side, Endosome, multivesicular body membrane, Peripheral membrane protein
Tissue Specificity Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
Database Links

HGNC: 4897

OMIM: 604375

KEGG: hsa:9146

STRING: 9606.ENSP00000331201

UniGene: Hs.661056

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