Human Homeobox protein NANOG(NANOG) ELISA kit

Code CSB-EL015424HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9H9S0
Lead Time 5-7 working days
Abbreviation NANOG
Protein Biological Process 1 Transcription/Transcription regulation
Target Name Nanog homeobox
Alias , homeobox transcription factor Nanog|homeobox transcription factor Nanog-delta 48
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 20857
RGD 1303178
MGI 1919200
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NANOG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %101
Range %96-103
1:2Average %94
Range %88-98
1:4Average %94
Range %89-97
1:8Average %92
Range %88-96
Recovery
The recovery of human NANOG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10297-105
EDTA plasma (n=4)8580-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.054 2.207 2.131 1.945
8001.391 1.492 1.442 1.256
4000.965 0.893 0.929 0.743
2000.601 0.618 0.610 0.424
1000.410 0.422 0.416 0.230
500.312 0.307 0.310 0.124
250.259 0.252 0.256 0.070
00.185 0.186 0.186
References
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Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter
Subcellular Location Nucleus
Protein Families Nanog homeobox family
Tissue Specificity Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and
Database Links

HGNC: 20857

OMIM: 607937

KEGG: hsa:79923

STRING: 9606.ENSP00000229307

UniGene: Hs.635882

Pathway Signaling pathways regulating pluripotency of stem cells

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