Human Hyaluronan mediated motility receptor(HMMR) ELISA kit

Code CSB-EL010582HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O75330
Lead Time 7-14 working days
Abbreviation HMMR
Target Name hyaluronan-mediated motility receptor (RHAMM)
Alias CD168, IHABP, MGC119494, MGC119495, RHAMM, hyaluronan-mediated motility receptor|intracellular hyaluronic acid binding protein
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
HGNC 5012
RGD 2805
MGI 104667
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HMMR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 98-111
1:2 Average % 98
Range % 89-102
1:4 Average % 103
Range % 93-107
1:8 Average % 104
Range % 95-108
Recovery
The recovery of human HMMR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 84-97
EDTA plasma (n=4) 100 88-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 1.968 1.912 1.940 1.767
5 1.529 1.507 1.518 1.345
2.5 1.035 1.001 1.018 0.845
1.25 0.750 0.735 0.743 0.570
0.625 0.458 0.448 0.453 0.280
0.312 0.333 0.321 0.327 0.154
0.156 0.244 0.250 0.247 0.074
0 0.177 0.169 0.173  
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Function Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).
Subcellular Location Cell surface, Cytoplasm
Tissue Specificity Expressed in breast cancer cell lines and in normal breast tissue.
Database Links

HGNC: 5012

OMIM: 600936

KEGG: hsa:3161

STRING: 9606.ENSP00000377492

UniGene: Hs.740467

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