Human Hypoxanthine-guanine phosphoribosyltransferase(HPRT1) ELISA kit

Code CSB-EL010706HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P00492
Lead Time 5-7 working days
Protein Biological Process 2 Alkaloid biosynthesis and metabolism
Abbreviation HPRT1
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name hypoxanthine phosphoribosyltransferase 1
Alias HGPRT, HPRT,
Species Homo sapiens (Human)
Protein Biological Process 3 Purine salvage
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 5157
RGD 2826
MGI 96217
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HPRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 103    
Range % 100-105    
1:2 Average % 89    
Range % 85-93    
1:4 Average % 102    
Range % 98-106    
1:8 Average % 85    
Range % 81-89    
Recovery
The recovery of human HPRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 99 95-106    
EDTA plasma (n=4) 87 83-90    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
10 2.082 2.101 2.092 2.005    
5 1.693 1.645 1.669 1.582    
2.5 1.242 1.301 1.272 1.185    
1.25 0.962 0.971 0.967 0.880    
0.625 0.597 0.568 0.583 0.496    
0.312 0.399 0.389 0.394 0.307    
0.156 0.275 0.281 0.278 0.191    
0 0.086 0.088 0.087      
References
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Function Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Involvement in disease Lesch-Nyhan syndrome (LNS); Gout HPRT-related (GOUT-HPRT)
Subcellular Location Cytoplasm
Protein Families Purine/pyrimidine phosphoribosyltransferase family
Database Links

HGNC: 5157

OMIM: 300322

KEGG: hsa:3251

STRING: 9606.ENSP00000298556

UniGene: Hs.412707

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