Human IgGFc-binding protein(FCGBP) ELISA kit

Code CSB-EL008536HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Fc fragment of IgG binding protein
Alternative Names FCGBPIgGFc-binding protein ELISA Kit; Fcgamma-binding protein antigen ELISA Kit; FcgammaBP ELISA Kit
Abbreviation FCGBP
Uniprot No. Q9Y6R7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human FCGBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 96
Range % 87-100
1:200 Average % 102
Range % 93-106
1:400 Average % 89
Range % 82-93
1:800 Average % 97
Range % 87-101
The recovery of human FCGBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 112 100-117
EDTA plasma (n=4) 98 90-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.831 2.714 2.773 2.652
2500 2.326 2.268 2.297 2.176
1250 1.544 1.579 1.562 1.441
625 0.792 0.759 0.776 0.655
312 0.422 0.411 0.417 0.296
156 0.267 0.251 0.259 0.138
78 0.181 0.176 0.179 0.058
0 0.122 0.120 0.121  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function May be involved in the maintenance of the mucosal structure as a gel-like component of the mucosa.
Gene References into Functions
  1. FCGBP, ubiquitous in vertebrates, has a conserved N-terminal domain. This domain is also present as an N-terminal sequence in a number of bacterial proteins. PMID: 27189557
  2. FCGBP may be used as a new biomarker for diagnosis, prevention or treatment for lung cancer. PMID: 24452952
  3. Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis. PMID: 24310606
  4. The GCTM-5 epitope on the mucin-like glycoprotein FCGBP is a cell surface marker for the study of normal differentiation lineages, regeneration, and disease progression in tissues of endodermal origin. PMID: 22761039
  5. The differentially expressed Fc gammaBP could reflect its potential role in prostate malignancy as well as an indicator for progression of the cancer. PMID: 17938577

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Subcellular Location Secreted
Tissue Specificity Mainly expressed in placenta and colon epithelium. Expressed in thyroid, and down-regulated in thyroid carcinomas. Present in serum, with higher levels in patients with various autoimmune diseases (at protein level).
Database Links

HGNC: 13572

OMIM: 617553

KEGG: hsa:8857

UniGene: Hs.111732


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