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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of human IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||93||87-101|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
This human IL1A ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL1A in multiple samples, including serum, plasma, cell culture supernates, tissue homogenates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of IL1A bound in the initial step.
IL1A is constitutively expressed in epithelial, endothelial, and stromal cells and can be upregulated in hematopoietic and non-hematopoietic cells by diverse stimuli, including TLR agonists, inflammatory cytokines, and oxidative stress. In non-hematopoietic cells, IL1A signals via IL1R on myeloid cells in a MyD88/RIPK1/(TAK1)-dependent manner to induce the expression of pro-inflammatory cytokines. IL1A also plays a role in neutrophil recruitment and host defense after infections caused by viral, fungal, or protozoan. Dysregulation of IL1A production is associated with numerous auto-inflammatory disorders.
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I am interested in ELISA Kit Cat#CSB-E04620h. My highest concentration of testing drug will contain 10% DMSO. I am worried the high concentration DMSO will affect the ELISA. Could you help to provide information about my worries? Thank you for your help.
My Experiment Procedure:
1. culture cell
2. Treat testing drug
3. Use supernates to run ELISA