Human Interleukin-1 receptor-like 2(IL1RL2) ELISA kit

Instructions
Code CSB-EL011627HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 1 receptor-like 2
Alternative Names IL 1 receptor related protein 2 ELISA Kit; IL 1Rrp2 ELISA Kit; IL 36R ELISA Kit; IL-1Rrp2 ELISA Kit; IL-36 receptor ELISA Kit; IL1 receptor related protein 2 ELISA Kit; IL1R rp2 ELISA Kit; IL1R-rp2 ELISA Kit; Il1rl2 ELISA Kit; IL1RRP2 ELISA Kit; IL36R ELISA Kit; ILRL2_HUMAN ELISA Kit; Interleukin 1 receptor like 2 ELISA Kit; Interleukin 1 receptor related protein 2 ELISA Kit; Interleukin-1 receptor-like 2 ELISA Kit; Interleukin-1 receptor-related protein 2 ELISA Kit
Abbreviation IL1RL2
Uniprot No. Q9HB29
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1RL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 94-102
1:2 Average % 86
Range % 83-89
1:4 Average % 92
Range % 89-95
1:8 Average % 101
Range % 98-104
Recovery
The recovery of human IL1RL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 83-90
EDTA plasma (n=4) 101 97-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.595 2.638 2.617 2.486
5 2.090 2.121 2.106 1.975
2.5 1.307 1.368 1.338 1.207
1.25 0.801 0.821 0.811 0.680
0.625 0.407 0.441 0.424 0.293
0.312 0.256 0.268 0.262 0.131
0.156 0.184 0.186 0.185 0.054
0 0.130 0.132 0.131  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways (By similarity). The IL-36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. Seems to be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway.
Gene References into Functions
  1. Endothelial cells, including dermal microvascular cells, express a functionally active IL-36 receptor. PMID: 27673278
  2. All human blood lymphocytes express IL-36R. PMID: 27269181
  3. The data identify a novel role for IL-36 signaling in colonic inflammation and indicate that the IL-36R pathway may represent a novel target for therapeutic intervention. PMID: 26813344
  4. single nucleotide polymorphism A471T in the Toll-interleukin 1 receptor domain (TIR) of the IL-1Rrp2 that is present in approximately 2% of the human population, down-regulated IL-36R signaling by a decrease of interaction with IL-1RAcP. PMID: 27307043
  5. The IL36 receptor (IL1RL2) is an important mediator molecule in the inflammatory response. PMID: 27853811
  6. The ubiquitin-binding Tollip protein contributed to IL-36R signaling and increased the accumulation of both subunits of the IL-36R. PMID: 26269592
  7. IL36A-IL36R axis is modulated in patients with primary Sjogren's syndrome. PMID: 25902739
  8. Expression of IL-1Rrp2 by sone dendritic cell subsets may have an important function in the human immune response in vivo via its role in differentiation of inflammatory Th1 lymphocytes. PMID: 22144259
  9. These data provide evidence that IL-38 binds to the IL-36R, as does IL-36Ra.[IL-38, IL-36R] PMID: 22315422
  10. IL-1alpha and IL-1beta gene polymorphisms do not act as a strong susceptibility factor for MTLE-HS in individuals of Turkish ancestry PMID: 16546408

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Interleukin-1 receptor family
Tissue Specificity Expressed in synovial fibroblasts and articular chondrocytes. Expressed in keratinocytes and monocyte-derived dendritic cells. Expressed in monocytes and myeloid dendritic cells; at protein level.
Database Links

HGNC: 5999

OMIM: 604512

KEGG: hsa:8808

STRING: 9606.ENSP00000264257

UniGene: Hs.659863

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