Human Interleukin-1 receptor-like 2(IL1RL2) ELISA kit

Code CSB-EL011627HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9HB29
Lead Time 7-14 working days
Abbreviation IL1RL2
Target Name interleukin 1 receptor-like 2
Alias IL1R-rp2, IL1RRP2, interleukin-1 receptor-related protein 2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
HGNC 5999
RGD 621782
MGI 1913107
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1RL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 94-102
1:2 Average % 86
Range % 83-89
1:4 Average % 92
Range % 89-95
1:8 Average % 101
Range % 98-104
Recovery
The recovery of human IL1RL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 83-90
EDTA plasma (n=4) 101 97-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.595 2.638 2.617 2.486
5 2.090 2.121 2.106 1.975
2.5 1.307 1.368 1.338 1.207
1.25 0.801 0.821 0.811 0.680
0.625 0.407 0.441 0.424 0.293
0.312 0.256 0.268 0.262 0.131
0.156 0.184 0.186 0.185 0.054
0 0.130 0.132 0.131  
Still Have Questions? Leave a Message or Start an on-line Chat
Function Receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways (By similarity). The IL-36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. Seems to be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway.
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Interleukin-1 receptor family
Tissue Specificity Expressed in synovial fibroblasts and articular chondrocytes. Expressed in keratinocytes and monocyte-derived dendritic cells. Expressed in monocytes and myeloid dendritic cells; at protein level.
Database Links

HGNC: 5999

OMIM: 604512

KEGG: hsa:8808

STRING: 9606.ENSP00000264257

UniGene: Hs.659863

Related Products

IL1RL2 Antibodies

IL1RL2 Antibodies for Human

IL1RL2 Proteins

IL1RL2 Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.