Human Interleukin 26,IL-26 ELISA Kit

Instructions
Code CSB-E11716h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Interleukin 26,IL-26
Alternative Names AK155 ELISA Kit; IL 26 ELISA Kit; IL-26 ELISA Kit; IL26 ELISA Kit; IL26_HUMAN ELISA Kit; Interleukin 26 ELISA Kit; Interleukin-26 ELISA Kit; Protein AK155 ELISA Kit
Abbreviation IL26
Uniprot No. Q9NPH9
Species Homo sapiens (Human)
Sample Types serum, urine, tissue homogenates, cell lysates, cell culture supernates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-102  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of human IL-26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 93-96  
EDTA plasma (n=4) 102 98-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.749 2.798 2.774 2.616  
2000 2.315 2.364 2.340 2.182  
1000 1.843 1.889 1.866 1.708  
500 1.399 1.381 1.390 1.232  
250 0.899 0.888 0.894 0.736  
125 0.483 0.487 0.485 0.327  
62.5 0.316 0.329 0.323 0.165  
0 0.157 0.159 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin.
Gene References into Functions
  1. this paper shows that IL-26 is overexpressed in Behcet's disease and enhances Th17 related -cytokines PMID: 28811236
  2. The varIL26 genotype is associated with reduced PMN capacity to kill bacteria. A varIL26 genotype is associated with decreased levels of anti-TNF-alpha in CD patients. IL26 may help explain the role of bactDNA as a risk factor of flare in CD patients. PMID: 28879509
  3. IL-26 activates STAT1/3 and leads to the induction of IL-6 and IL-8 expression in non-transformed cells derived from human colon. PMID: 28852311
  4. IL-26 levels are higher in synovial fluid compared to plasma in spondyloarthritis. IL-26 was identified in axial facet joints of spondyloarthritis patients. Myofibroblasts from the spondyloarthritis synovium produce large amounts of IL-26. IL-26 induces bone mineralization in human osteoblasts. PMID: 28365787
  5. this study shows that IL-26 is a unique cationic protein more similar to a soluble pattern recognition receptor than to conventional cytokines PMID: 28356384
  6. this study shows that single nucleotide polymorphism near IL-26 gene is associated with familial vitiligo and existence of halo nevi in Estonian patients PMID: 26429320
  7. Our study indicates that IL-26 is a potential biomarker of disease severity in pediatric asthma without signs of Th2-mediated inflammation. PMID: 27029915
  8. IL-26 is emerging as a potentially important player in host defense and may also be a pathogenic factor in the chronic inflammatory disorders of humans. [Review] PMID: 26202572
  9. The negative influence of IL-26 on the anti-mycobacterial activity and its constitutive presence in both serum and monocyte supernatants prompt a proposal that IL26 as a candidate gene for tuberculosis susceptibility. PMID: 25157980
  10. IL-26 differentially modulates the infection by different enveloped viruses. PMID: 23875025
  11. Elevated levels of IL-26 in human gastric cancer promote proliferation and survival by modulating STAT1/STAT3 signaling. PMID: 23704922
  12. IL-26 appears as a novel proinflammatory cytokine, located upstream of the proinflammatory cascade, that may constitute a promising target to treat rheumatoid arthritis and chronic inflammatory disorders. PMID: 23055831
  13. Identify a second enhancer element positioned between IL26 and IFNG required for both IL26 and IFNG expression. One function of this enhancer is to facilitate recruitment of RNA polymerase II to promoters of both genes. PMID: 22622197
  14. common polymorphisms in the IFNgamma/IL-26 gene region may contribute to sex bias in susceptibility to rheumatoid arthritis, by distorting the propensity of female carriers versus male carriers to contract this disease. PMID: 14558082
  15. The active receptor complex for IL-26 is a heterodimer composed of two receptor proteins: IL-20 receptor 1 and IL-10 receptor 2. Signaling through the IL-26 receptor results in activation of STAT1 and STAT3. PMID: 14764663
  16. sensitivity to recombinant interleukin-26(IL-26) of various cell lines strictly correlated with the expression of IL-20 receptor 1 and blocking antibodies against either IL-10 receptor 2 or IL-20 receptor 1 inhibited IL-26-dependent signal transduction PMID: 15178681

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Subcellular Location Secreted
Protein Families IL-10 family
Tissue Specificity Expressed in HVS transformed T-cells but not other T-cell lines or primary stimulated T-cells. Expressed in colonic T-cells including Th17 inflammatory T-cells; the expression is significantly increased in serum of patients with Crohn's disease (at protei
Database Links

HGNC: 17119

OMIM: 605679

KEGG: hsa:55801

STRING: 9606.ENSP00000229134

UniGene: Hs.272350

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