Human Interleukin 4 receptor (IL4R/CD124) ELISA Kit

Code CSB-E13936h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 4 receptor
Alternative Names CD124, IL4RA, OTTHUMP00000122506|interleukin 4 receptor alpha chain|interleukin-4 receptor alpha chain
Abbreviation IL4R
Uniprot No. P24394
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human IL4R/CD124 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 90-104
1:2 Average % 94
Range % 87-99
1:4 Average % 90
Range % 84-95
1:8 Average % 104
Range % 98-108
The recovery of human IL4R/CD124 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 90-103
EDTA plasma (n=4) 97 91-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.003 1.942 1.973 1.836
5 1.496 1.453 1.475 1.338
2.5 0.927 0.946 0.937 0.800
1.25 0.619 0.595 0.607 0.470
0.625 0.439 0.430 0.435 0.298
0.312 0.307 0.314 0.311 0.174
0.156 0.244 0.237 0.241 0.104
0 0.136 0.138 0.137  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Subcellular Location Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Secreted
Protein Families Type I cytokine receptor family, Type 4 subfamily
Tissue Specificity Isoform 1 and isoform 2 are highly expressed in activated T-cells.
Database Links

HGNC: 6015

OMIM: 147781

KEGG: hsa:3566

STRING: 9606.ENSP00000170630

UniGene: Hs.513457

Most popular with customers


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 CUSABIO TECHNOLOGY LLC All Rights Reserved.