Human Junctional adhesion molecule B(JAM2) ELISA kit

Unavailable
Code CSB-EL011936HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P57087
Lead Time 7-14 working days
Abbreviation JAM2
Target Name junctional adhesion molecule 2
Alias C21orf43, CD322, JAM-B, JAMB, PRO245, VE-JAM, VEJAM, JAM-IT/VE-JAM|junctional adhesion molecule B|vascular endothelial junction-associated molecule
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.
HGNC 14686
RGD 1561692
MGI 1933820
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human JAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 106
Range % 101-114
1:4 Average % 87
Range % 82-91
1:8 Average % 93
Range % 89-97
Recovery
The recovery of human JAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 80-88
EDTA plasma (n=4) 96 91-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 2.743 2.678 2.711 2.544
4000 2.081 2.002 2.042 1.875
2000 1.497 1.423 1.460 1.293
1000 0.926 0.945 0.936 0.769
500 0.542 0.567 0.555 0.388
250 0.319 0.304 0.312 0.145
125 0.275 0.281 0.278 0.111
0 0.164 0.169 0.167  
Still Have Questions? Leave a Message or Start an on-line Chat
Function May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Subcellular Location Cell junction, tight junction, Cell membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily
Tissue Specificity Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
Database Links

HGNC: 14686

OMIM: 606870

KEGG: hsa:58494

STRING: 9606.ENSP00000420419

UniGene: Hs.517227

Pathway Leukocyte transendothelial migration

Related Products

JAM2 Antibodies

JAM2 Antibodies for Human

JAM2 Antibodies for Homo sapiens (Human)

JAM2 Proteins

JAM2 Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.