Human Laminin subunit alpha-4(LAMA4) ELISA kit

Instructions
Code CSB-EL012728HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name laminin, alpha 4
Alternative Names CMD1JJ ELISA Kit; DKFZp686D23145 ELISA Kit; LAMA3 ELISA Kit; LAMA3, formerly ELISA Kit; LAMA4 1 ELISA Kit; LAMA4 ELISA Kit; LAMA4*-1 ELISA Kit; LAMA4_HUMAN ELISA Kit; Laminin alpha 4 ELISA Kit; Laminin alpha 4 chain ELISA Kit; Laminin subunit alpha 4 precursor ELISA Kit; Laminin subunit alpha-4 ELISA Kit; Laminin-14 subunit alpha ELISA Kit; Laminin-8 subunit alpha ELISA Kit; Laminin-9 subunit alpha ELISA Kit
Abbreviation LAMA4
Uniprot No. Q16363
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LAMA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 95
Range % 88-99
1:40 Average % 104
Range % 98-108
1:80 Average % 90
Range % 85-94
1:160 Average % 99
Range % 90-103
Recovery
The recovery of human LAMA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 84-97
EDTA plasma (n=4) 101 93-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.673 2.586 2.630 2.535
750 2.180 2.145 2.163 2.068
375 1.786 1.712 1.749 1.654
187.5 1.201 1.239 1.220 1.125
94 0.653 0.648 0.651 0.556
47 0.415 0.422 0.419 0.324
23.5 0.252 0.263 0.258 0.163
0 0.093 0.097 0.095  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Gene References into Functions
  1. High LAMA4 expression is associated with gastric cancer. PMID: 30015861
  2. Disruption of LAMA4, LAMA5, and LAMB2 or the laminin-receptor interaction occurs in neuromuscular diseases including Pierson syndrome and Lambert-Eaton myasthenic syndrome (LEMS). (Review) PMID: 27614294
  3. highlight MCAM and LAMA4 as prime candidates for RCC prognosis and therapeutic targeting PMID: 26921326
  4. LAMA4-integrin signalling affects chondrocyte morphology and gene expression, contributing to cluster formation in osteoarthritic chondrocytes. PMID: 26295200
  5. LAMA4 expression is lowered in preeclamptic placentas and promotes trophoblast cell invasion, migration, and angiogenesis. hypoxia-reoxygenation decreases LAMA4 expression and decouple the relationship between LAMA4 expression and p38 and ERK activation. PMID: 26059342
  6. Oxidative stress plays a vital role in controlling expression of LAMA4 through MAPK signaling pathways, which suggests a possible pathological mechanism of pre-eclampsia. PMID: 25676580
  7. Laminins 411 and 421 differentially promote tumor cell migration via alpha6beta1 integrin and MCAM (CD146). PMID: 24951930
  8. Data from experiment with peptide fragments suggest laminin alpha4 (and laminin alpha5) may be part of host defense response and may protect tissues from invading pathogens. PMID: 20433883
  9. Laminins with alpha1, alpha4, and alpha5 chains are compared to determine laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. PMID: 12393739
  10. Data report the identification of a splice variant of the human laminin alpha4 subunit transcript containing 21 extra nucleotides. PMID: 12445830
  11. The G domain of laminin alpha4 chain is a specific, high affinity ligand for alphavbeta3 & alpha3beta1 integrin heterodimers. These integrins, with alpha6beta1, cooperatively mediate endothelial cell-alpha4 laminin interaction & blood vessel development. PMID: 12454288
  12. The alpha4 laminin subunit regulates vascular endothelium cell survival. PMID: 14980521
  13. laminin alpha4 LG4 module may play an important role in cell adhesion and/or vessel wall formation in the skin by interacting with syndecan-2 and/or -4. PMID: 15086543
  14. Downregulation of laminin alpha4 chain expression inhibits glioma invasion PMID: 15915502
  15. laminin isoform changes are associated with brain tumor invasion and angiogenesis [review] PMID: 16146715
  16. This suggests that G(1121-1139) peptide-containing proteins may perform their biological functions by interacting with alphavbeta3 integrin. PMID: 16824487
  17. alpha4 chain Lms have a de-adhesive function and could thus play a role in detachment, migration and invasion of renal carcinoma cells in vivo. PMID: 17533363
  18. 2 novel mutations (2828C>T [Pro943Leu] & 3217C>T [Arg1073X]) in the integrin-interacting domain of the LAMA4 gene reduce its integrin binding, & cause endothelial cell loss and heart failure. PMID: 17646580
  19. LAMA4 is specifically up-regulated on both mRNA and protein levels in hepatocelluar carcinoma; potential role in hepatocarcinogenesis and tumor progression. PMID: 18084776
  20. Concomitant changes take place in laminin- and collagen-binding receptors. Laminin-411 reduces adhesion to laminin-511 and fibronectin, suggesting that tumor cells could utilize laminin-411 in their invasive behavior PMID: 18496706
  21. LM alpha4 and beta2 have roles in in vitro migration and in vivo tumorigenicity of prostate cancer cells PMID: 19048114

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Involvement in disease Cardiomyopathy, dilated 1JJ (CMD1JJ)
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Specificity In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn t
Database Links

HGNC: 6484

OMIM: 600133

KEGG: hsa:3910

STRING: 9606.ENSP00000230538

UniGene: Hs.654572

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