Human Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1(LINGO1) ELISA kit

Instructions
Code CSB-EL012967HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leucine rich repeat and Ig domain containing 1
Alternative Names FLJ14594 ELISA Kit; LERN 1 ELISA Kit; LERN1 ELISA Kit; Leucine rich repeat and Ig domain containing 1 ELISA Kit; Leucine rich repeat neuronal 6A ELISA Kit; Leucine rich repeat neuronal protein 1 ELISA Kit; Leucine rich repeat neuronal protein 6A ELISA Kit; Leucine-rich repeat and immunoglobilin domain-containing protein 1 ELISA Kit; Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 ELISA Kit; Leucine-rich repeat neuronal protein 1 ELISA Kit; Leucine-rich repeat neuronal protein 6A ELISA Kit; LIGO1_HUMAN ELISA Kit; Lingo 1 ELISA Kit; LINGO1 ELISA Kit; LRR and Ig domain containing Nogo Receptor interating protein ELISA Kit; Lrrn 6a ELISA Kit; Lrrn6a ELISA Kit; Lrrn6a protein ELISA Kit; MGC17422 ELISA Kit; Nogo Receptor interacting protein ELISA Kit; PRO227 ELISA Kit; UNQ201 ELISA Kit
Abbreviation LINGO1
Uniprot No. Q96FE5
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LINGO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 91-99
1:2 Average % 87
Range % 83-91
1:4 Average % 102
Range % 98-106
1:8 Average % 85
Range % 81-90
Recovery
The recovery of human LINGO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-98
EDTA plasma (n=4) 103 100-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml. OD1 OD2 Average Corrected
20 2.079 2.104 2.092 1.964
10 1.634 1.689 1.662 1.534
5 1.120 1.202 1.161 1.033
2.5 0.681 0.698 0.690 0.562
1.25 0.454 0.461 0.458 0.330
0.625 0.292 0.287 0.290 0.162
0.312 0.236 0.224 0.230 0.102
0 0.127 0.129 0.128  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors
Gene References into Functions
  1. We identified homozygous missense variants in LINGO1, p.(Arg290His) in family F162 and p.(Tyr288Cys) in family PKMR65. Both variants were predicted to be pathogenic, and segregated with the phenotype in the respective families. Molecular modeling of LINGO1 suggests that both variants interfere with the glycosylation of the protein. PMID: 28837161
  2. LINGO1 rs11856808 plays a protective role by decreasing the risk for PD, but not for MSA, in Chinese population. PMID: 26254004
  3. LINGO1 protein acts as a gateway protein internalizing into the tumor cells when engaged by antibody and can carry antibody conjugated with drugs to kill Ewing sarcoma cells. PMID: 26979953
  4. The results of this study show Increased LINGO1 in the cerebellum of essential tremor patients. PMID: 24531928
  5. Lingo-1 signaling is altered in the schizophrenia brain. PMID: 24448210
  6. LINGO-1 can directly bind to ErbB2, block ErbB2 translocation into lipid rafts, and inhibit its phosphorylation. PMID: 24583087
  7. This review found that several gene variants in the LINGO1 gene may increase the risk of essential tremor. PMID: 23682623
  8. LINGO1 variants are associated with essential tremor in Chinese Han female patients. PMID: 23754655
  9. This study demonistrated that theLINGO1 rs9652490 and rs11856808 polymorphisms are not associated with risk for multiple sclerosis. PMID: 23574883
  10. Results of a meta-analysis suggest a relationship between LINGO1 rs11856808 polymorphism and risk of essential tremor (ET) and familial ET, while rs9652490 polymorphism is only associated with the risk for familial ET. PMID: 22425540
  11. the N-terminal region containing the leucine-rich repeats along with the transmembrane and cytoplasmic domains of LINGO-1 are not required for self-interaction or interaction with amyloid precursor protein PMID: 22133804
  12. the present meta-analysis does not support the notion that LINGO1 rs9652490 SNP is a major genetic risk factor for parkinson disease.[meta-analysis] PMID: 22710005
  13. This study showed that LINGO1 rs9652490 and rs11856808 are not associated with the risk of Parkinson's disease. PMID: 21955595
  14. LINGO-1, a transmembrane signaling protein, inhibits oligodendrocyte differentiation and myelination through intercellular self-interactions PMID: 22514275
  15. There were no significant differences in frequencies for all alleles between the essential tremor (ET) group and controls; however, an association of genotype A/G of the SNP rs9652490 with the familial ET phenotype was found. PMID: 21741293
  16. Single nucleotide polymorphism(SNP)s of LINGO1 play a role in the development of Parkinson's disease in the Italian population. PMID: 21752692
  17. LINGO1 variants are not a major risk factor for developing familial essential tremor in this population, which suggests the existence of other genetic risk factors responsible for familial essential tremor in this movement disorder clinic population. PMID: 21219542
  18. while there were no significant differences in the Lingo1 minor allele frequency and genotype frequency between Chinese essential tremor and controls, pooled analysis showed a higher proportion of GG genotype in tremor patients PMID: 21158743
  19. No significant differences are found in genotype and allele distribution in the rs9652490 variant of LINGO1 in either Chinese or Caucasian Parkinson disease patients. PMID: 20951767
  20. This study demonistrated that LINGO1 variants could increase risk of PD, specifically those presenting the non-rigid-akinetic phenotypes, which suggests that LINGO1 may have a role in the etiology of tremor in PD at least in the Spanish population. PMID: 21506150
  21. genotyping results lead us to conclude that no association exists between the key variant rs9652490 and Essential tremor (P(corr) = 1.00). PMID: 21264305
  22. LINGO1 variant rs9652490 (A > G) is unlikely to play a major role in Parkinson's disease in Chinese populations. PMID: 20957646
  23. LINGO-1 inhibits multiple aspects of oligodendrocyte differentiation independently of the LRRs via a process that requires p75(NTR) signalling PMID: 20659559
  24. LINGO1 and LINGO2 variants are associated with essential tremor and Parkinson disease PMID: 20369371
  25. The two markers rs9652490 and rs11856808 were not strongly related to the essential tremor,late onset sporadic Parkinson's disease, but the rs9652490G allele might be a protective factor for the early onset Parkinson's disease in Chinese population. PMID: 20600614
  26. The LINGO1 gene is a risk factor for essential tremor in a Caucasian population in North America. PMID: 20372186
  27. Our study gives further evidence that LINGO1 acts as a susceptibility gene for essential tremor. PMID: 20310002
  28. Thisdy demonstrateda significant association between LINGO1 rs9652490 and essential tremor (P = 0.014) and Parkinson disease (P = 0.0003), thus providing the first evidence of a genetic link between both diseases. PMID: 19720553
  29. LINGO1 SNP (rs9652490) is not associated with sporadic PD in our Polish cohort. PMID: 20117178
  30. In this study, SNPs rs9652490, rs11856808, and rs7177008 of LINGO1 were genotyped in a total of 694 Austrian subjects (349 PD, 345 controls). No association could be found between genotype or allele counts and Parkinson disease. PMID: 19908305
  31. The LERN1 expression pattern is specific to the central nervous system, highly and broadly expressed during early stages of development and gradually restricted to forebrain structures as development proceeds(LERN1) PMID: 14686891
  32. the Lingo-1 ectodomain is a module implicated in central nervous system repair inhibition PMID: 17005555
  33. inhibitory agents of LINGO-1 activity can protect dopaminergic neurons against degeneration PMID: 17726113
  34. Variant in the sequence of the LINGO1 gene confers risk of essential tremor. PMID: 19182806
  35. Quick identification of essential N-glycosylation sites of a heavily glycosylated neuroglycoprotein Lingo-1, which are sufficient for the support of its surface expression. PMID: 19254717
  36. the LINGO1 gene is associated with an increased risk for essential tremor PMID: 19553813
  37. LINGO1 variant increases risk of familial essential tremor. PMID: 19805735

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Tissue Specificity Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkins
Database Links

HGNC: 21205

OMIM: 609791

KEGG: hsa:84894

STRING: 9606.ENSP00000347451

UniGene: Hs.656765

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