Human Lysosome-associated membrane glycoprotein 1(LAMP1) ELISA kit

Code CSB-EL012738HU
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P11279
Lead Time 7-14 working days
Abbreviation LAMP1
Target Name lysosomal-associated membrane protein 1
Alias CD107a, LAMPA, LGP120,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
HGNC 6499
RGD 2989
MGI 96745
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LAMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-95
1:2 Average % 101
Range % 98-104
1:4 Average % 96
Range % 90-102
1:8 Average % 92
Range % 87-96
Recovery
The recovery of human LAMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-106
EDTA plasma (n=4) 85 82-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.331 2.156 2.244 2.143
5 1.933 1.968 1.951 1.850
2.5 1.372 1.302 1.337 1.236
1.25 0.702 0.738 0.720 0.619
0.625 0.307 0.312 0.310 0.209
0.312 0.226 0.219 0.223 0.122
0.156 0.187 0.181 0.184 0.083
0 0.100 0.101 0.101  
Still Have Questions? Leave a Message or Start an on-line Chat
Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.; FUNCTION
Subcellular Location Cell membrane, Single-pass type I membrane protein, Endosome membrane, Single-pass type I membrane protein, Lysosome membrane, Single-pass type I membrane protein, Late endosome
Protein Families LAMP family
Database Links

HGNC: 6499

OMIM: 153330

KEGG: hsa:3916

STRING: 9606.ENSP00000333298

UniGene: Hs.494419

Pathway Autophagy
Secreted Extracellular Vesicles

Related Products

LAMP1 Antibodies

LAMP1 Antibodies for Homo sapiens (Human)

LAMP1 Proteins

LAMP1 Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.