Human Lysosome-associated membrane glycoprotein 1(LAMP1) ELISA kit

Instructions
Code CSB-EL012738HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name lysosomal-associated membrane protein 1
Alternative Names CD107a, LAMPA, LGP120,
Abbreviation LAMP1
Uniprot No. P11279
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LAMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-95
1:2 Average % 101
Range % 98-104
1:4 Average % 96
Range % 90-102
1:8 Average % 92
Range % 87-96
Recovery
The recovery of human LAMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-106
EDTA plasma (n=4) 85 82-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.331 2.156 2.244 2.143
5 1.933 1.968 1.951 1.850
2.5 1.372 1.302 1.337 1.236
1.25 0.702 0.738 0.720 0.619
0.625 0.307 0.312 0.310 0.209
0.312 0.226 0.219 0.223 0.122
0.156 0.187 0.181 0.184 0.083
0 0.100 0.101 0.101  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
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Target Data

Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.; FUNCTION
Subcellular Location Cell membrane, Single-pass type I membrane protein, Endosome membrane, Single-pass type I membrane protein, Lysosome membrane, Single-pass type I membrane protein, Late endosome
Protein Families LAMP family
Database Links

HGNC: 6499

OMIM: 153330

KEGG: hsa:3916

STRING: 9606.ENSP00000333298

UniGene: Hs.494419

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