Human Mothers against decapentaplegic homolog 6(SMAD6) ELISA kit

Unavailable
Code CSB-EL021792HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O43541
Lead Time 5-7 working days
Abbreviation SMAD6
Protein Biological Process 1 Transcription/Transcription regulation
Target Name SMAD family member 6
Alias HsT17432, MADH6, MADH7, Mothers against decapentaplegic, drosophila, homolog of, 6|SMAD, mothers against DPP homolog 6
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 6772
RGD 1305069
MGI 1336883
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SMAD6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 829-91
1:2 Average % 98
Range % 94-104
1:4 Average % 92
Range % 87-98
1:8 Average % 101
Range % 95-105
Recovery
The recovery of human SMAD6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 82-94
EDTA plasma (n=4) 94 86-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 1.886 1.945 1.916 1.750
500 1.351 1.427 1.389 1.223
250 0.929 0.948 0.939 0.773
125 0.631 0.644 0.638 0.472
62.5 0.421 0.432 0.427 0.261
31.2 0.296 0.286 0.291 0.125
15.6 0.231 0.237 0.234 0.068
0 0.165 0.166 0.166  
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Function Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.
Involvement in disease Aortic valve disease 2 (AOVD2); Craniosynostosis 7 (CRS7)
Subcellular Location Nucleus
Protein Families Dwarfin/SMAD family
Tissue Specificity Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
Database Links

HGNC: 6772

OMIM: 602931

KEGG: hsa:4091

STRING: 9606.ENSP00000288840

UniGene: Hs.153863

Pathway TGF-beta signaling pathway

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