Human NKG2-D type II integral membrane protein(KLRK1) ELISA kit

Instructions
Code CSB-EL012474HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name killer cell lectin-like receptor subfamily K, member 1
Alternative Names CD314 ELISA Kit; CD314 antigen ELISA Kit; D12S2489E ELISA Kit; Killer cell lectin like receptor subfamily K member 1 ELISA Kit; Killer cell lectin-like receptor subfamily K member 1 ELISA Kit; KLR ELISA Kit; KLRC4 KLRK1 readthrough ELISA Kit; KLRK1 ELISA Kit; NK cell receptor D ELISA Kit; NK lectin-like receptor ELISA Kit; NKG2 D activating NK receptor ELISA Kit; NKG2 D type II integral membrane protein ELISA Kit; NKG2-D ELISA Kit; NKG2-D type II integral membrane protein ELISA Kit; NKG2-D-activating NK receptor ELISA Kit; Nkg2d ELISA Kit; NKG2D_HUMAN ELISA Kit; NKLLR ELISA Kit; NKR P2 ELISA Kit; Nkrp2 ELISA Kit
Abbreviation KLRK1
Uniprot No. P26718
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KLRK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-91
1:2 Average % 94
Range % 89-98
1:4 Average % 107
Range % 103-110
1:8 Average % 96
Range % 90-100
Recovery
The recovery of human KLRK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 91-99
EDTA plasma (n=4) 103 98-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.774 2.768 2.771 2.672
10 1.956 1.932 1.944 1.845
5 1.208 1.300 1.254 1.155
2.5 0.684 0.702 0.693 0.594
1.25 0.428 0.431 0.430 0.331
0.625 0.308 0.312 0.310 0.211
0.312 0.213 0.223 0.218 0.119
0 0.098 0.100 0.099  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
Subcellular Location Cell membrane, Single-pass type II membrane protein
Tissue Specificity Expressed in natural killer (NK) cells, CD8(+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).
Database Links

HGNC: 18788

OMIM: 611817

KEGG: hsa:100528032

STRING: 9606.ENSP00000240618

UniGene: Hs.387787

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