Human Neudesin(NENF) ELISA kit

Instructions
Code CSB-EL015711HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name neuron derived neurotrophic factor
Alternative Names Cell growth inhibiting protein 47 ELISA Kit; Cell immortalization-related protein 2 ELISA Kit; CIR2 ELISA Kit; Nenf ELISA Kit; NENF_HUMAN ELISA Kit; Neudesin ELISA Kit; Neuron derived neurotrophic factor ELISA Kit; Neuron-derived neurotrophic factor ELISA Kit; SCIRP10 ELISA Kit; SCIRP10 related protein ELISA Kit; Secreted protein of unknown function ELISA Kit; Spinal cord injury related protein 10 ELISA Kit; SPUF ELISA Kit; SPUF protein ELISA Kit
Abbreviation NENF
Uniprot No. Q9UMX5
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NENF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-104
1:2Average %91
Range %82-98
1:4Average %94
Range %90-98
1:8Average %87
Range %82-96
Recovery
The recovery of human NENF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-98
EDTA plasma (n=4)9489-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.698 2.752 2.725 2.570
52.382 2.364 2.373 2.218
2.51.887 1.965 1.926 1.771
1.251.355 1.272 1.314 1.159
0.6250.766 0.764 0.765 0.610
0.3120.475 0.453 0.464 0.309
0.1560.272 0.289 0.281 0.126
00.153 0.157 0.155  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis
Gene References into Functions
  1. High NENF expression is associated with liver cancer. PMID: 24763612
  2. Human neudesin of 172 amino acids with high similarity ( approximately 91% identity) to mouse neudesin was identified; the human neudesin gene was mapped to chromosome 1p33. PMID: 15605373
  3. Describes the predicted heme-binding domains in the human neudesin protein sequence. PMID: 18056703
Subcellular Location Secreted, extracellular space
Protein Families Cytochrome b5 family, MAPR subfamily
Tissue Specificity Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.
Database Links

HGNC: 30384

OMIM: 611874

KEGG: hsa:29937

STRING: 9606.ENSP00000355955

UniGene: Hs.461787

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