Human Peptidyl-prolyl cis-trans isomerase FKBP10(FKBP10) ELISA kit

Unavailable
Code CSB-EL008687HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q96AY3
Lead Time 7-14 working days
Abbreviation FKBP10
Target Name FK506 binding protein 10, 65 kDa
Alias FKBP65, FLJ20683, FLJ22041, FLJ23833, PPIASE, hFKBP65, FK506 binding protein 10|immunophilin FKBP65|peptidyl-prolyl cis-trans isomerase|rotamase
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 18169
RGD 1549751
MGI 104769
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FKBP10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 92-107
1:2 Average % 97
Range % 91-103
1:4 Average % 95
Range % 88-99
1:8 Average % 89
Range % 85-93
Recovery
The recovery of human FKBP10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 97-105
EDTA plasma (n=4) 93 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 1.987 2.029 2.008 1.874
1000 1.424 1.495 1.460 1.326
500 0.924 0.996 0.960 0.826
250 0.633 0.629 0.631 0.497
125 0.364 0.377 0.371 0.237
62.5 0.241 0.259 0.250 0.116
31.25 0.182 0.189 0.186 0.052
0 0.133 0.134 0.134  
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Function PPIases accelerate the folding of proteins during protein synthesis.
Involvement in disease Osteogenesis imperfecta 11 (OI11); Bruck syndrome 1 (BRKS1)
Subcellular Location Endoplasmic reticulum lumen
Database Links

HGNC: 18169

OMIM: 259450

KEGG: hsa:60681

STRING: 9606.ENSP00000317232

UniGene: Hs.463035

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