Human Phosphatidylinositol 3-kinase regulatory subunit beta(PIK3R2) ELISA kit

Unavailable
Code CSB-EL018005HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O00459
Lead Time 7-14 working days
Abbreviation PIK3R2
Target Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Alias P85B, p85, p85-BETA, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)|phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)|phosphoinositide-3-kinase, regulatory subu
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 8980
RGD 68341
MGI 1098772
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PIK3R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %89-98
1:2Average %98
Range %93-103
1:4Average %95
Range %89-99
1:8Average %94
Range %87-98
Recovery
The recovery of human PIK3R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-102
EDTA plasma (n=4)8782-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.352 2.559 2.456 2.312
7501.593 1.494 1.544 1.400
3750.912 0.875 0.894 0.750
187.50.562 0.577 0.570 0.426
93.750.345 0.354 0.350 0.206
46.880.257 0.262 0.260 0.116
23.440.199 0.193 0.196 0.052
00.143 0.144 0.144
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Function Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy
Involvement in disease Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 (MPPH1)
Protein Families PI3K p85 subunit family
Database Links

HGNC: 8980

OMIM: 603157

KEGG: hsa:5296

STRING: 9606.ENSP00000471914

UniGene: Hs.371344

Pathway cAMP signaling pathway
Chemokine signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
HIF-1 signaling pathway
Jak-STAT signaling pathway
mTOR signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
TNF signaling pathway
Apoptosis
Autophagy
Cellular senescence
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
B cell receptor signaling pathway
Fc epsilon RI signaling pathway
Fc gamma R-mediated phagocytosis
Leukocyte transendothelial migration
Natural killer cell mediated cytotoxicity
Osteoclast differentiation
T cell receptor signaling pathway
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
FoxO signaling pathway
Insulin signaling pathway
Phosphatidylinositol signaling system
Phospholipase D signaling pathway
Rap1 signaling pathway
Sphingolipid signaling pathway
Neurotrophin signaling pathway

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