Human Pikachurin(EGFLAM) ELISA kit

Instructions
Code CSB-EL007478HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name EGF-like, fibronectin type III and laminin G domains
Alternative Names Agrin-like protein ELISA Kit; AGRINL ELISA Kit; AGRNL ELISA Kit; EGF-like ELISA Kit; EGF-like; fibronectin type III and laminin G domains ELISA Kit; EGF-like; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; EGFLA_HUMAN ELISA Kit; EGFLAM ELISA Kit; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; FLJ39155 ELISA Kit; Pikachurin ELISA Kit
Abbreviation EGFLAM
Uniprot No. Q63HQ2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EGFLAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-102
1:2Average %85
Range %81-92
1:4Average %96
Range %90-102
1:8Average %93
Range %87-99
Recovery
The recovery of human EGFLAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9895-104
EDTA plasma (n=4)10298-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.717 2.787 2.752 2.644
10002.120 2.160 2.140 2.032
5001.468 1.425 1.447 1.339
2500.798 0.769 0.784 0.676
1250.469 0.457 0.463 0.355
62.50.290 0.280 0.285 0.177
31.250.212 0.225 0.219 0.111
00.107 0.109 0.108  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness (By similarity).
Gene References into Functions
  1. Strong candidate gene for macular dystrophy, MCDR3 (human 5p15.33-p13.1). Conclusion is based on a massive expression data set for mouse (103 strains) and joint analysis of RetNet database. PMID: 19727342
Subcellular Location Secreted, extracellular space, extracellular matrix, Cell junction, synapse
Database Links

HGNC: 26810

OMIM: 617683

KEGG: hsa:133584

STRING: 9606.ENSP00000346964

UniGene: Hs.20103

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