Human Protein APCDD1(APCDD1) ELISA kit

Instructions
Code CSB-EL001896HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name adenomatosis polyposis coli down-regulated 1
Alternative Names Adenomatosis polyposis coli down regulated 1 ELISA Kit; Adenomatosis polyposis coli down regulated 1 protein ELISA Kit; Adenomatosis polyposis coli down-regulated 1 protein ELISA Kit; APCD1_HUMAN ELISA Kit; APCDD 1 ELISA Kit; APCDD1 ELISA Kit; B7323 ELISA Kit; DRAPC1 ELISA Kit; FP7019 ELISA Kit; HHS ELISA Kit; HTS ELISA Kit; hypoptrichosis simplex ELISA Kit; Protein APCDD1 ELISA Kit
Abbreviation APCDD1
Uniprot No. Q8J025
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human APCDD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %84-96
1:2Average %102
Range %95-106
1:4Average %92
Range %82-98
1:8Average %93
Range %85-97
Recovery
The recovery of human APCDD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9286-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.431 2.283 2.357 2.191
25001.468 1.398 1.433 1.267
12500.826 0.892 0.859 0.693
6250.538 0.528 0.533 0.367
3120.374 0.353 0.364 0.198
1560.278 0.272 0.275 0.109
780.228 0.224 0.226 0.060
00.166 0.165 0.166
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Negative regulator of the Wnt signaling pathway. Inhibits Wnt signaling in a cell-autonomous manner and functions upstream of beta-catenin. May act via its interaction with Wnt and LRP proteins. May play a role in colorectal tumorigenesis.
Gene References into Functions
  1. Thus, we have provided the first evidence that APCDD1 expression is epigenetically silenced in OS, which may facilitate invasion and metastasis of OS cells. PMID: 28698141
  2. these novel findings suggest that APCDD1 positively regulates adipogenic differentiation and that its down-regulation by miR-130 during diet-induced obesity may contribute to impaired adipogenic differentiation and obesity-related metabolic disease. PMID: 28242765
  3. This study demonstrated a critical role for Apcdd1 in OL differentiation after white matter injury that points to a potential therapeutic approach for inhibiting Wnt signaling in these disorders. PMID: 25946682
  4. Unusual role of APCDD1 in dental follicle cells during osteogenic differentiation. APCDD1 sustains the expression and activation of beta-catenin. PMID: 25592970
  5. mutation in the APCDD1 gene is responsible for hereditary hypotrichosis simplex in a large Chinese family. PMID: 22512811
  6. Data show that the methylated VAPA-APCDD1 DNA in maternal plasma is predominantly derived from the fetus, and this novel fetal epigenetic marker in maternal plasma is useful for the noninvasive detection of fetal trisomy 18. PMID: 21152411
  7. APCDD1 is a novel inhibitor of the Wnt signalling pathway with an essential role in human hair growth PMID: 20393562

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Involvement in disease Hypotrichosis 1 (HYPT1)
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families APCDD1 family
Tissue Specificity Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes. Abundantly expressed in both the epidermal and dermal compartments of the hair follicle. Present in
Database Links

HGNC: 15718

OMIM: 605389

KEGG: hsa:147495

STRING: 9606.ENSP00000347433

UniGene: Hs.293274

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