Human Protein FEV(FEV) ELISA kit

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Code CSB-EL008599HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q99581
Lead Time 5-7 working days
Abbreviation FEV
Protein Biological Process 1 Developmental Protein
Target Name FEV (ETS oncogene family)
Alias HSRNAFEV, PET-1, FEV (fifth Ewing variant)
Species Homo sapiens (Human)
Protein Biological Process 3 Differentiation
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39.07 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations.
HGNC 18562
RGD 628860
MGI 2449712
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FEV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 81-88
1:2 Average % 93
Range % 92-98
1:4 Average % 101
Range % 95-108
1:8 Average % 96
Range % 90-102
Recovery
The recovery of human FEV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 95-104
EDTA plasma (n=4) 85 81-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.474 2.574 2.524 2.357
1250 1.635 1.688 1.662 1.495
625 0.999 0.968 0.984 0.817
312.5 0.560 0.544 0.552 0.385
156.25 0.354 0.332 0.343 0.176
78.13 0.281 0.285 0.283 0.116
39.07 0.225 0.241 0.233 0.066
0 0.163 0.170 0.167  
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Function Functions as a transcriptional regulator. According to PubMed
Involvement in disease Sudden infant death syndrome (SIDS)
Subcellular Location Nucleus
Protein Families ETS family
Tissue Specificity In brain, exclusively expressed in the major serotonergic neurons of the dorsal and median raphe nuclei located in the midbrain and pons. Also detected in prostate and small intestine.
Database Links

HGNC: 18562

OMIM: 272120

KEGG: hsa:54738

STRING: 9606.ENSP00000295727

UniGene: Hs.234759

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