Human Protein cereblon(CRBN) ELISA kit

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Code CSB-EL005944HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q96SW2
Lead Time 5-7 working days
Abbreviation CRBN
Protein Biological Process 1 Ubiquitin
Target Name cereblon
Alias DKFZp781K0715, MGC27358, MRT2A, mental retardation, non-syndromic, autosomal recessive, 2A|protein x 0001
Species Homo sapiens (Human)
Protein Biological Process 3 Ubl conjugation pathway
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 37.5 pg/mL-2400 pg/mL
Sensitivity 9.38 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 30185
RGD 1310533
MGI 1913277
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CRBN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 88-97
1:2 Average % 100
Range % 91-105
1:4 Average % 94
Range % 86-98
1:8 Average % 99
Range % 94-102
Recovery
The recovery of human CRBN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-95
EDTA plasma (n=4) 98 91-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2400 2.429 2.368 2.399 2.240
1200 1.615 1.542 1.579 1.420
600 0.844 0.799 0.822 0.663
300 0.490 0.473 0.482 0.323
150 0.322 0.314 0.318 0.159
75 0.258 0.251 0.255 0.096
37.5 0.201 0.195 0.198 0.039
0 0.161 0.157 0.159  
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Function Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
Involvement in disease Mental retardation, autosomal recessive 2A (MRT2A)
Subcellular Location Cytoplasm, Nucleus, Membrane, Peripheral membrane protein
Protein Families CRBN family
Tissue Specificity Widely expressed. Highly expressed in brain.
Database Links

HGNC: 30185

OMIM: 607417

KEGG: hsa:51185

STRING: 9606.ENSP00000231948

UniGene: Hs.18925

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