Human Ras-related protein Rab-5B(RAB5B) ELISA kit

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Code CSB-EL019214HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P61020
Lead Time 5-7 working days
Abbreviation RAB5B
Protein Biological Process 1 Transport
Target Name RAB5B, member RAS oncogene family
Species Homo sapiens (Human)
Protein Biological Process 3 Protein transport
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 9784
RGD 1306489
MGI 105938
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RAB5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %81-89
1:2Average %98
Range %95-102
1:4Average %96
Range %93-99
1:8Average %104
Range %101-107
Recovery
The recovery of human RAB5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9895-102
EDTA plasma (n=4)9390-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.484 2.469 2.477 2.316
7501.711 1.735 1.723 1.562
3751.144 1.165 1.155 0.994
187.50.706 0.736 0.721 0.560
93.750.476 0.498 0.487 0.326
46.880.359 0.369 0.364 0.203
23.440.238 0.249 0.244 0.083
00.160 0.162 0.161
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Function Protein transport. Probably involved in vesicular traffic (By similarity).
Subcellular Location Cell membrane, Lipid-anchor, Cytoplasmic side, Early endosome membrane, Lipid-anchor, Melanosome
Protein Families Small GTPase superfamily, Rab family
Database Links

HGNC: 9784

OMIM: 179514

KEGG: hsa:5869

STRING: 9606.ENSP00000353444

UniGene: Hs.157659

Pathway Ras signaling pathway
Endocytosis

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