Human Receptor activity-modifying protein 1(RAMP1) ELISA kit

Unavailable
Code CSB-EL019304HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O60894
Lead Time 5-7 working days
Abbreviation RAMP1
Protein Biological Process 1 Transport
Target Name receptor (G protein-coupled) activity modifying protein 1
Alias , calcitonin receptor-like receptor activity modifying protein 1|receptor (calcitonin) activity modifying protein 1|receptor activity modifying protein 1|receptor activity-modifying protein 1
Species Homo sapiens (Human)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28.12 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface.
HGNC 9843
RGD 61870
MGI 1858418
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RAMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 91-107
1:2 Average % 100
Range % 91-104
1:4 Average % 99
Range % 88-103
1:8 Average % 91
Range % 83-94
Recovery
The recovery of human RAMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 86-97
EDTA plasma (n=4) 94 85-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1800 2.058 1.944 2.001 1.887
900 1.301 1.276 1.289 1.175
450 0.834 0.819 0.827 0.713
225 0.556 0.545 0.551 0.437
112.5 0.337 0.325 0.331 0.217
56.25 0.232 0.238 0.235 0.121
28.12 0.154 0.161 0.158 0.044
0 0.113 0.115 0.114  
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Function Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL.
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families RAMP family
Tissue Specificity Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract.
Database Links

HGNC: 9843

OMIM: 605153

KEGG: hsa:10267

STRING: 9606.ENSP00000254661

UniGene: Hs.471783

Pathway Vascular smooth muscle contraction

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