Human Receptor activity-modifying protein 1(RAMP1) ELISA kit

Instructions
Code CSB-EL019304HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name receptor (G protein-coupled) activity modifying protein 1
Alternative Names , calcitonin receptor-like receptor activity modifying protein 1|receptor (calcitonin) activity modifying protein 1|receptor activity modifying protein 1|receptor activity-modifying protein 1
Abbreviation RAMP1
Uniprot No. O60894
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28.12 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RAMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 91-107
1:2 Average % 100
Range % 91-104
1:4 Average % 99
Range % 88-103
1:8 Average % 91
Range % 83-94
Recovery
The recovery of human RAMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 86-97
EDTA plasma (n=4) 94 85-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1800 2.058 1.944 2.001 1.887
900 1.301 1.276 1.289 1.175
450 0.834 0.819 0.827 0.713
225 0.556 0.545 0.551 0.437
112.5 0.337 0.325 0.331 0.217
56.25 0.232 0.238 0.235 0.121
28.12 0.154 0.161 0.158 0.044
0 0.113 0.115 0.114  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL.
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families RAMP family
Tissue Specificity Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract.
Database Links

HGNC: 9843

OMIM: 605153

KEGG: hsa:10267

STRING: 9606.ENSP00000254661

UniGene: Hs.471783

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.