Human Rhodopsin(RHO) ELISA kit

Code CSB-EL019681HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08100
Lead Time 5-7 working days
Abbreviation RHO
Protein Biological Process 1 Neurobiology
Target Name rhodopsin
Alias CSNBAD1, MGC138309, MGC138311, OPN2, RP4, opsin 2, rod pigment|retinitis pigmentosa 4, autosomal dominant|rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
Species Homo sapiens (Human)
Protein Biological Process 3 Sensory transduction
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness.
HGNC 30655
RGD 708493
MGI 2444891
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %80-100
1:2Average %95
Range %92-105
1:4Average %95
Range %90-107
1:8Average %97
Range %85-110
Recovery
The recovery of human RHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-104
EDTA plasma (n=4)9490-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
8002.322 2.174 2.248 1.989
4001.940 1.860 1.900 1.641
2001.331 1.342 1.337 1.078
1000.927 0.955 0.941 0.682
500.632 0.598 0.615 0.356
250.418 0.432 0.425 0.166
12.50.352 0.341 0.347 0.088
00.254 0.264 0.259  
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Function Photoreceptor required for image-forming vision at low light intensity
Involvement in disease Retinitis pigmentosa 4 (RP4); Night blindness, congenital stationary, autosomal dominant 1 (CSNBAD1)
Subcellular Location Membrane, Multi-pass membrane protein, Cell projection, cilium, photoreceptor outer segment
Protein Families G-protein coupled receptor 1 family, Opsin subfamily
Tissue Specificity Rod shaped photoreceptor cells which mediate vision in dim light.
Database Links

HGNC: 10012

OMIM: 180380

KEGG: hsa:6010

STRING: 9606.ENSP00000296271

UniGene: Hs.247565

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