Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3(ATP2A3) ELISA kit

Instructions
Code CSB-EL002334HU
Size
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Product Details

Target Name ATPase, Ca++ transporting, ubiquitous
Alternative Names Adenosine triphosphatase calcium ELISA Kit; AT2A3_HUMAN ELISA Kit; Atp2a3 ELISA Kit; ATPase Ca(2+) transporting ubiquitous ELISA Kit; ATPase Ca++ transporting ubiquitous ELISA Kit; Calcium pump 3 ELISA Kit; Calcium translocating P type ATPase ELISA Kit; Sarco/endoplasmic reticulum Ca2+ ATPase ELISA Kit; Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 ELISA Kit; SERCA 3 ELISA Kit; SERCA3 ELISA Kit; SERCA3b ELISA Kit; SR Ca(2+) ATPase 3 ELISA Kit; SR Ca(2+)-ATPase 3 ELISA Kit
Abbreviation ATP2A3
Uniprot No. Q93084
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP2A3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 93-104
1:2 Average % 97
Range % 91-103
1:4 Average % 93
Range % 89-98
1:8 Average % 87
Range % 81-92
Recovery
The recovery of human ATP2A3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 86 82-91
EDTA plasma (n=4) 97 93-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 1.822 1.918 1.870 1.689
800 1.365 1.462 1.414 1.233
400 0.974 0.997 0.986 0.805
200 0.666 0.684 0.675 0.494
100 0.442 0.457 0.450 0.269
50 0.332 0.316 0.324 0.143
25 0.237 0.244 0.241 0.060
0 0.180 0.181 0.181  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Gene References into Functions
  1. Sp1, Sp3, and Klf-4 transcription factors bind to ATP2A3 proximal promoter elements and regulate basal gene expression. The study showed that these factors participated in the increase of ATP2A3 expression during cancer cell differentiation. PMID: 27433831
  2. Together, these data provide evidence for the interaction of Bcl-2 with SERCA3b in vitro and in cell culture, and for Bcl-2-dependent conformational and functional changes of SERCA3b. PMID: 27639965
  3. ATP2A3 methylation was not altered between patients with type 2 diabetes and healthy men. Moreover, a glucose challenge did not alter ATP2A3 methylation. PMID: 28011458
  4. we make a comprehensive analysis of the current knowledge of the role of the SERCA pumps in the pathophysiology of insulin-dependent diabetes mellitus type 1 (TIDM) and type 2 (T2DM) in the heart and beta-cells in the pancreas PMID: 25270119
  5. Normal choroid plexus epithelial cells express SERCA3 abundantly, SERCA3 expression is strongly decreased in papillomas, and is absent in choroid plexus carcinoma, while expression in hyperplastic epithelium is high, similarly to normal epithelium. PMID: 24224513
  6. aberrant SERCA3 expression is closely linked to the adenoma-adenocarcinoma sequence and progression of colorectal carcinomas. PMID: 24213720
  7. The Ca2+-ATPase activity in cloned Plasmodiuim falciparum strain D6 protein is higher than in cloned strain W2 protein. The Ca2+-ATPase activity in isolates from malaria patients varied widely. PMID: 23235306
  8. Loss of SERCA3 expression is associated with lung cancer. PMID: 23157274
  9. High SERCA3 expression is associated with pathogenesis, invasion, metastasis of gastric carcinomas. PMID: 22948776
  10. novel sarco/endoplasmic isoforms; data suggest that the SERCA3 gene products have a more widespread role in cellular Ca2+ signaling than previously appreciated PMID: 11956212
  11. SERCA3 constitutes an interesting new differentiation marker that may prove useful for the analysis of the phenotype of gastrointestinal adenocarcinomas. PMID: 11986315
  12. Results describe the catalytic cycle of three human SERCA3 isoenzymes: SERCA3a, b, and c. PMID: 12207029
  13. A method is described for a calcium-activated ATPase technique to be peformed. PMID: 12503734
  14. monoclonal antibody PL/IM430 inhibits SERCA3 activity by sterically preventing movement of the actuator domain into a catalytically critical position in the E2 conformation of the enzyme. PMID: 12540840
  15. SERCA3 isoforms have a more widespread role in cellular Ca(2+) signaling: analysis of SERCA3f isoform PMID: 15028735
  16. link SERCA3 expression to the state of differentiation of colonic epithelial cells PMID: 15972967
  17. By increasing the rate of Ca2+ sequestration, up-regulation of SERCA 3 counteracts the cytosolic increase in Ca2+ concentration. PMID: 16250893
  18. Data showed that overexpression of SERCA3b affected cell adhesion and SERCA3f overexpression resulted in an increase in endoplasmic reticulum stress markers. PMID: 16725111
  19. Ca2+ ATPase sarcoplasmic/endoplasmic reticulum calcium ATPase 3 are involved in an increased susceptibility to develop head and neck squamous cell carcinoma in humans. PMID: 18295663
  20. These findings revisit the human heart's Ca(2+)ATPase system and indicate that SERCA3f may account for the mechanism of endoplasmic reticulum stress in vivo in heart failure. PMID: 18947868
  21. ATP2A3 gene is involved in cancer susceptibility. PMID: 19100511
  22. two distinct SERCA3 fragmentation profiles sign the co-expression of SERCA3 proteins in two conformational states in platelet membranes. PMID: 19135027
  23. affinity for Ca(2+) is inherently lower for SERCA3 expressed in situ than for other SERCA isoforms PMID: 19225163

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Subcellular Location Nucleus membrane, Multi-pass membrane protein, Endoplasmic reticulum membrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIA subfamily
Tissue Specificity Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic orig
Database Links

HGNC: 813

OMIM: 601929

KEGG: hsa:489

STRING: 9606.ENSP00000353072

UniGene: Hs.513870

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