Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

Code CSB-EL013017HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
lemur tyrosine kinase 3
Alternative Names
LMTK3 ELISA Kit; KIAA1883 ELISA Kit; TYKLM3 ELISA Kit; Serine/threonine-protein kinase LMTK3 ELISA Kit; EC 2.7.11.1 ELISA Kit; Lemur tyrosine kinase 3 ELISA Kit
Abbreviation
LMTK3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
18.75 pg/mL-1200 pg/mL
Sensitivity
4.7 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LMTK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 97
Range % 92-101
1:4 Average % 89
Range % 84-93
1:8 Average % 94
Range % 90-98
Recovery
The recovery of human LMTK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-98
EDTA plasma (n=4) 105 100-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 2.687 2.532 2.610 2.450
600 1.994 2.018 2.006 1.846
300 1.269 1.295 1.282 1.122
150 0.716 0.728 0.722 0.562
75 0.475 0.483 0.479 0.319
37.5 0.321 0.307 0.314 0.154
18.75 0.243 0.239 0.241 0.081
0 0.158 0.162 0.160  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human LMTK3 ELISA Kit was designed for the quantitative measurement of Human LMTK3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

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Target Background

Function
(From Uniprot)
Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription. Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons.
Subcellular Location
Membrane; Single-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Golgi apparatus membrane. Note=Punctate pattern in cell projections.
Protein Families
Protein kinase superfamily, Tyr protein kinase family
Database Links

HGNC: 19295

UniGene: Hs.207426

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