Human Solute carrier family 35 member D3(SLC35D3) ELISA kit

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Code CSB-EL021596HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q5M8T2
Lead Time 5-7 working days
Abbreviation SLC35D3
Protein Biological Process 1 Transport
Target Name solute carrier family 35, member D3
Alias FRCL1, MGC102873, bA55K22.3, frc, fringe-like 1
Species Homo sapiens (Human)
Protein Biological Process 3 Sugar transport
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.67 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 15621
RGD 1309428
MGI 1923407
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SLC35D3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %80-92
1:2Average %90
Range %85-85
1:4Average %92
Range %88-98
1:8Average %104
Range %100-108
Recovery
The recovery of human SLC35D3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9993-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.596 2.412 2.504 2.375
6001.731 1.846 1.789 1.660
3001.164 1.194 1.179 1.050
1500.703 0.679 0.691 0.562
750.428 0.454 0.441 0.312
37.50.307 0.289 0.298 0.169
18.750.212 0.209 0.211 0.082
00.126 0.131 0.129
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Function May play a role in hemostasis as a regulator of the biosynthesis of platelet-dense granules.
Subcellular Location Membrane, Multi-pass membrane protein
Protein Families TPT transporter family, SLC35D subfamily
Database Links

HGNC: 15621

OMIM: 612519

KEGG: hsa:340146

STRING: 9606.ENSP00000333591

UniGene: Hs.369703

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