Human Spectrin alpha chain, brain(SPTAN1) ELISA kit

Instructions
Code CSB-EL022633HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
Alternative Names (ALPHA)II-SPECTRIN ELISA Kit; Alpha II Spectrin ELISA Kit; Alpha-II spectrin ELISA Kit; brain ELISA Kit; EIEE5 ELISA Kit; FLJ17738 ELISA Kit; FLJ44613 ELISA Kit; Fodrin alpha chain ELISA Kit; Fodrin, alpha ELISA Kit; NEAS ELISA Kit; Non erythrocytic spectrin alpha ELISA Kit; non-erythroid alpha chain ELISA Kit; SPECA ELISA Kit; Spectrin alpha chain ELISA Kit; Spectrin alpha chain brain ELISA Kit; Spectrin alpha non erythrocytic 1 ELISA Kit; Spectrin ELISA Kit; Spectrin non erythroid alpha chain ELISA Kit; Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) ELISA Kit; Spectrin, nonerythroid, alpha subunit ELISA Kit; Spna2 ELISA Kit; SPTA 2 ELISA Kit; SPTA2 ELISA Kit; SPTA2_HUMAN ELISA Kit; SPTAN 1 ELISA Kit; SPTAN1 ELISA Kit
Abbreviation SPTAN1
Uniprot No. Q13813
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SPTAN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %88-94
1:2Average %97
Range %94-100
1:4Average %101
Range %96-106
1:8Average %89
Range %81-97
Recovery
The recovery of human SPTAN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9088-94
EDTA plasma (n=4)10299-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.435 2.650 2.543 2.386
102.045 2.220 2.133 1.976
51.421 1.544 1.483 1.326
2.50.856 0.859 0.858 0.701
1.250.613 0.638 0.626 0.469
0.6250.407 0.430 0.419 0.262
0.3120.273 0.288 0.281 0.124
00.151 0.162 0.157
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Gene References into Functions
  1. Spectrin may be engaged in regulation of distinct events necessary for the establishment and maturity of the immunological synapse. PMID: 29244882
  2. SPTAN1-related disorders comprise a wide spectrum of neurodevelopmental phenotypes ranging from mild to severe and progressive. Spectrin aggregate formation in fibroblasts with mutations in the alpha/beta heterodimerization domain seems to be associated with a severe neurodegenerative course. PMID: 29050398
  3. Alpha-spectrin is critical for recruitment of non-ubiquitinated FANCD2 to sites of damage, which has an important role in the repair response and interstrand cross-link repair. PMID: 26297932
  4. Studies demonstrate that alpha-IISp plays a critical role in maintaining chromosome stability in cells after DNA interstrand cross-links damage by repairing damage that occurs in both genomic and telomeric DNA. PMID: 25757157
  5. These results suggest that ubiquitin C-terminal hydrolase and alphaII-spectrin breakdown product 145 kDa may be useful in assessing outcome after pediatric traumatic brain injury. PMID: 22022780
  6. Variations in both alpha-spectrin (SPTA1) and beta-spectrin ( SPTB ) were found in a neonate with prolonged jaundice in a family where nine individuals had hereditary elliptocytosis. PMID: 24193021
  7. aggressiveness of MLH1-positive colorectal cancers might be related to SPTAN1. PMID: 24456667
  8. hypomyelination of the cerebral white matter was revealed at the age of 1 year, and a de novo heterozygous mutation in the SPTAN1 gene was confirmed PMID: 22656320
  9. loss of SPTAN1 switches TGF-beta signaling from tumor suppression to tumor promotion by engaging Notch signaling and activating SOX9 in esophageal adenocarcinoma. PMID: 23536563
  10. organization of a spectrin-like cytoskeleton is associated with keratinocyte differentiation, and cytoskeleton disruption is mediated by either PKCdelta(Thr505) phosphorylation associated with phosphorylated adducin or due to reduction of endogenous adducin PMID: 22163289
  11. In-frame mutations in the C-terminus of SPTAN1 cause a core set of manifestations that include severe intellectual disability, generalized epilepsy, and pontocerebellar atrophy. PMID: 22258530
  12. Analysis of alphaII-spectrin breakdown products in cerebrospinal fluid predicts mortality and injury severity in adults following traumatic brain injury. PMID: 20408766
  13. findings suggest that pathological aggregation of alpha/beta spectrin heterodimers and abnormal axon initial segment(AIS) integrity resulting from SPTAN1 mutations were involved in pathogenesis of infantile epilepsy PMID: 20493457
  14. Fanconi anemia proteins may play an important role in maintaining the stability of alphaIISp in the cell by regulating its cleavage by mu-calpain. PMID: 20518497
  15. This protein has been found differentially expressed in the anterior cingulate cortex from patients with schizophrenia PMID: 20381070
  16. SigA binds to epithelial HEp-2 cells as well as being able to induce fodrin degradation in vitro and in situ, further extending its documented role in the pathogenesis of Shigella infections. PMID: 20011051
  17. Review. 120 kDa alpha-fodrin is an important autoantigen in both animal models and Sjogren syndrome patients. Increased caspase cascade activity may be involved in the progression of alpha-fodrin proteolysis and tissue destruction. PMID: 12630566
  18. High affinity and slow overall kinetics of association/dissociation of alpha II-spectrin may suit it well to a role in strengthening cell junctions and providing stable anchorage for transmembrane proteins at points specified by cell-adhesion molecules. PMID: 12820899
  19. Fanconi anemia cell lines deficient in alphaII spectrin express normal levels of alphaII spectrin mRNA. PMID: 12893251
  20. These observations indicate that calpain is activated and reacts with alpha-fodrin as a substrate at the sarcolemma, and plays a key role in modulating sarcolemmal proteins to adapt to the specific conditions in each myopathy. PMID: 15948206
  21. The in vivo role of alpha-fodrin autoantigen in primary Sjogren's syndrome was analyzed. PMID: 16192640
  22. These studies indicate that alphaSpIISigma( *) may play a role in a number of diverse and important processes in the nucleus and that a deficiency in this protein, as occurs in Fanconi's anemia, could affect a number of critical cellular pathways. PMID: 16889989
  23. provide novel insights into spectrin functions by demonstrating the involvement of alphaII-spectrin in cell cycle regulation and actin organization PMID: 18978357
  24. The SH3 domain of SPTAN1 is a target for the Fanconi anemia protein, FANCG. PMID: 19102630
  25. depletion of alphaIISp in normal cells leads to a number of defects observed in Fanconi anemia cells, such as chromosome instability and a deficiency in cross-link repair. PMID: 19217883
  26. fodrin degradation occurs during galectin-1 T cell death and CD45 is essential for fodrin degradation to occur PMID: 19454697
  27. This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. PMID: 19034380

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Involvement in disease Epileptic encephalopathy, early infantile, 5 (EIEE5)
Subcellular Location Cytoplasm, cytoskeleton, Cytoplasm, cell cortex
Protein Families Spectrin family
Database Links

HGNC: 11273

OMIM: 182810

KEGG: hsa:6709

STRING: 9606.ENSP00000361824

UniGene: Hs.372331

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