Human Sperm surface protein Sp17(SPA17) ELISA kit

Instructions
Code CSB-EL022451HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name sperm autoantigenic protein 17
Alternative Names Band 34 ELISA Kit; Cancer/testis antigen 22 ELISA Kit; CT22 ELISA Kit; SP17 1 ELISA Kit; Sp17-1 ELISA Kit; SP17_HUMAN ELISA Kit; SPA17 ELISA Kit; Sperm autoantigenic protein 17 ELISA Kit; Sperm protein 17 ELISA Kit; Sperm surface protein Sp17 ELISA Kit
Abbreviation SPA17
Uniprot No. Q15506
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 64 pg/mL-2400 pg/mL
Sensitivity 16 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SPA17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 103  
Range % 98-109  
1:2 Average % 90  
Range % 85-94  
1:4 Average % 84  
Range % 80-88  
1:8 Average % 89  
Range % 84-93  
Recovery
The recovery of human SPA17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-101  
EDTA plasma (n=4) 95 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2400 2.401 2.257 2.329 2.284  
1280 1.812 1.682 1.747 1.702  
640 1.076 1.053 1.065 1.020  
160 0.321 0.345 0.333 0.288  
64 0.175 0.167 0.171 0.126  
0 0.044 0.046 0.045    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity).
Gene References into Functions
  1. Sp17 and ASP cancer/testis antigens were found in ciliated cells of four ciliated hepatic foregut cysts (CHFCs). Further characterization of Sp17 and ASP in patients with CHFCs may provide significant clues for understanding the molecular mechanisms underlying their predisposition to develop squamous cell carcinomas. PMID: 25600306
  2. dendritic cells from human umbilical cord blood modulated for SP17 expression induced antigen-specific anti-tumor immunity against SP17(+) non-small cell lung cancer. PMID: 26300426
  3. SP17/AKAP4/PTTG1 are expressed in both human NSCLC cell lines and primary tumors and can elicit an immunogenic response in lung cancer patients. PMID: 25739119
  4. NY-ESO-1 and SP17 was not significantly associated with a specific histotype, but high-level GAGE expression was more frequent in squamous cell carcinoma. GAGE expression was demonstrated to be significantly higher in stage II-IIIa than stage I NSCLC. PMID: 24103781
  5. Sp17 is highly expressed in hepatocellular carcinoma cells PMID: 23923079
  6. SP17 was expressed in many cancer types with an overall frequency of 12%. SP17 was most frequently expressed in a different set of cancer types than MAGE-A and GAGE antigens and rarely overlapped with these proteins. PMID: 23137323
  7. Sperm protein 17 is highly expressed in endometrial and cervical cancers. PMID: 20712874
  8. Data show that cisplatin induced decrease in Sp17 levels was due to transcriptional inhibition and cisplatin-resistant cell lines did not show this decrease in Sp17 levels in response to cisplatin treatment. PMID: 19685492
  9. Sp17 has different expression patterns in cancer cell lines as compared to normal non-testes tissues and a potential pathogenic role in diseased cells PMID: 12213290
  10. focuses on the characterization of the genomic organization of an intron-containing gene and establishes a new transcription model PMID: 12393185
  11. expression of Sp17, a thought to be gamete-specific protein, in the synoviocytes of 8/8 female rheumatoid arthritis patients PMID: 12739786
  12. identification of a nonapeptide sequence within the Sp17 protein that is predicted to have a high binding affinity for HLA-A1 molecules PMID: 14566839
  13. Sp17 is expressed in the cell nucleus of ovarian neoplasms PMID: 14712480
  14. Sp17 gene expression in myeloma cells is regulated by promoter methylation PMID: 15381930
  15. Overexpression of sperm protein 17 is associated with esophageal cancer PMID: 17230514
  16. in vitro cultured, monoclonal, cytotoxic T lymphocytes (derived either from advanced OC patients or from healthy donors), specific for sperm protein 17, can eradicate human metastatic OC cells. PMID: 18779750
  17. Results suggest a possible role of Sp17 in regulating sperm maturation, capacitation, acrosomal reaction and interactions with the oocyte zona pellucida during the fertilization process. PMID: 19604394
  18. HSp17 plays a role in metastatic disease and resistance of epithelial ovarian carcinoma to chemotherapy PMID: 19744347

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Subcellular Location Membrane, Peripheral membrane protein
Tissue Specificity Testis and sperm specific.
Database Links

HGNC: 11210

OMIM: 608621

KEGG: hsa:53340

STRING: 9606.ENSP00000227135

UniGene: Hs.286233

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