This human SOD2 ELISA Kit is suitable for qualitatively determining human concentrations in serum, plasma, cell culture supernates, cell lysates, or tissue homogenates in vitro. SOD2 is a mitochondrial-specific antioxidant enzyme that dismutates superoxide to hydrogen peroxide, which is then converted to water by catalase and glutathione peroxidase. SOD2 is synthesized in the cytoplasm and localizes to the mitochondrial matrix, where it scavenges and accelerates the dismutation of superoxide anion generated by respiratory chain enzymes. It also plays a crucial role in the maintenance of vascular function. SOD2 overexpression and excess generation of hydrogen peroxide have been involved in angiogenesis by enhancing oxidation of phosphatase and tensin homolog deleted from chromosome 10, cell differentiation through prolonged ERK1/2 signaling, as well as pulmonary hypertension through the activation of hypoxia-inducible factor 1α-O2–sensitive pathways. A deficiency of SOD2 in apolipoprotein E–deficient mice leads to mitochondrial damage and impaired vascular relaxation in response to acetylcholine.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human SOD2 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human SOD2 antibody. Biotin-labeled SOD2 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the SOD2 in the human samples. SOD2 binds to the biotinylated anti-human human monoclonal antibody. And the biotin on the biotinylated anti-SOD2 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of SOD2 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.