Human Superoxide dismutase [Mn], mitochondrial (SOD2) ELISA kit

Instructions
Code CSB-E17064h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name superoxide dismutase 2, mitochondrial
Alternative Names Indophenoloxidase B ELISA Kit; IPO B ELISA Kit; IPOB ELISA Kit; Manganese containing superoxide dismutase ELISA Kit; Manganese SOD ELISA Kit; Manganese superoxide dismutase ELISA Kit; Mangano superoxide dismutase ELISA Kit; Mn SOD ELISA Kit; Mn superoxide dismutase ELISA Kit; MNSOD ELISA Kit; MVCD6 ELISA Kit; SOD 2 ELISA Kit; SOD2 ELISA Kit; SODM_HUMAN ELISA Kit; Superoxide dismutase [Mn] mitochondrial ELISA Kit; Superoxide dismutase [Mn], mitochondrial ELISA Kit; Superoxide dismutase 2 mitochondrial ELISA Kit
Abbreviation SOD2
Uniprot No. P04179
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %88
Range %80-92
1:400Average %95
Range %91-100
1:800Average %100
Range %90-110
1:1600Average %93
Range %86-98
Recovery
The recovery of human SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9390-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.248 2.152 2.200 2.107
5001.967 1.890 1.929 1.836
2501.685 1.604 1.645 1.552
1251.334 1.319 1.327 1.234
62.50.925 0.904 0.915 0.822
31.20.629 0.590 0.610 0.517
15.60.277 0.269 0.273 0.180
00.096 0.090 0.093  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Gene References into Functions
  1. Mn-SOD V allele carries a worse outcome profile after stroke, relating to nitrosative stress, inflammatory, and apoptotic response and associated with a BDNF reduction. PMID: 30150066
  2. Master athletes had lower levels of miR-7, while mRNA or protein levels of SIRT3, SIRT1, SOD2, and FOXO1 levels were significantly higher in the vastus lateralis muscle of master athletes compared to muscles of age-matched controls. PMID: 30107294
  3. These findings revealed the role of forkhead box M1 upregulation by manganese superoxide dismutase overexpression in maintaining lung cancer stem-like cell properties. Therefore, inhibition of forkhead box M1 upregulation by manganese superoxide dismutase overexpression may represent an effective therapeutic strategy for non-small cell lung cancer. PMID: 30111255
  4. genetic association studies in population of men in Brazil: Data suggest that an SNP in SOD2 (rs4880, Val16Ala) is associated with sperm quality and male infertility in the population studied; lipid peroxidation appears higher in homozygous versus heterozygous sperm samples. PMID: 28658993
  5. High SOD2 expression can be predictive of a poor clinical outcome and be clinically useful in the follow-up of metastatic Renal cell carcinoma (RCC). Therapeutics for metastatic RCCs require further improvement, such as supplementary administration of agents targeting mitochondrial SOD2 PMID: 29914010
  6. Low SOD2 expression is associated with childhood obesity. PMID: 29953407
  7. GLUT1 ectopic overexpression makes PCa cells more resistant to glucose deprivation and oxidative stress-induced cell death. Under glucose deprivation, GLUT1 overexpressing PCa cells sustains mitochondrial SOD2 activity, compromised after glucose removal, and significantly increases reduced glutathione (GSH) PMID: 29684818
  8. Dual presence of the SOD2 Ala16Val TT variant among mother-father pairs was associated with an increased risk of pre-eclampsia when compared with the absence of the TT variant among the mother-father pairs. The SOD2 Ala16Val SNP might be involved in paternal influence on the maternal predisposition to pre-eclampsia. PMID: 29745991
  9. These findings uncover a novel role for SOD2 in regulating RIG-I-like receptor -mediated antiviral innate immune signaling. PMID: 28574756
  10. Diabetic ESRD carriers of CC genotype of SOD2 exon 2 had an increased risk of mortality. PMID: 27925431
  11. The single nucleotide polymorphism rs4880 of the SOD2 gene was not correlated with male infertility, which, however, is to be supported by further studies with larger samples from more areas. PMID: 29658251
  12. MiR-509-5p exerted tumor-suppressive effects on breast cancer progression and metastasis via targeting SOD2 in vitro. PMID: 28925482
  13. review: discuss how H2O2 formation in mitochondria depends on and is controlled by MnSOD PMID: 28616679
  14. SOD2 gene polymorphism may modulate biochemical responses to a 12-week swimming training. PMID: 29109056
  15. Study shows an association of the polymorphism of MnSOD 47C/T in the Algerian population with type 1 diabetes without complications. PMID: 29859283
  16. MnSOD is the major superoxide scavenger in mitochondria, whose activity is regulated by SIRT3-mediated deacetylation, particularly at the Lys68 site, and can be enhanced by a SIRT3 coumarin derivative. PMID: 28711502
  17. Heavy alcohol drinking down-regulates ALDH2 gene expression level. Heavy smoking up-regulates SOD2 gene expression level in patients with head and neck squamous cell carcinoma. PMID: 28841898
  18. This is the first report evaluating the role of SOD2 in native and T351-mutated BCR-ABL-expressing cells and in a large cohort of chronic myeloid leukemia patients. In leukemic cells silenced for SOD2 expression a specific down-regulation of the expression of PRDX2 gene was found. PMID: 29550484
  19. The investigated MnSOD and Catalase polymorphisms do not predispose to the development of alcoholic Chronic Pancreatitis. PMID: 28655148
  20. Allele-specific interaction between GPX1 and MnSOD affects the levels of Bcl2, Sirt3 and E-cadherin. PMID: 28587495
  21. Studies suggest that both SOD3 and SOD2 superoxide dismutases are regulated by oxidative stress and redox-dependent signaling mechanisms. PMID: 29047081
  22. Data confirmed that SOD2 is important in the development and prognosis of salivary adenoid cystic carcinoma (SACC); that SOD2 deregulation is related to migration and invasion in SACC; and that the SOD2-dependent production of intracellular H2O2 promotes the migration and invasion of SACC, which involves the ERK-Slug signaling pathway. PMID: 27181103
  23. A model for electrostatic-mediated diffusion, and efficient binding of superoxide for catalysis is presented. PMID: 28461152
  24. This study builds upon in vivo investigation examined the heroin dependency risk associated with the rs2758339 and rs5746136 polymorphisms, as well as their relationship with the SOD2 mRNA levels in normal peripheral blood cells. The findings of the present study indicated that the study polymorphisms are significantly associated with the risk of dependency to heroin but are not associated with the SOD2 expression level. PMID: 29459008
  25. The results of this study revealed higher radial diffusivity in the anterior thalamic radiation among SOD2 CC genotypes compared to CT/TT genotypes. PMID: 28457881
  26. Val 16Ala polymorphism in SOD2 gene contributes to individual variability in oxidative stress status and lipid profile of the blood in young wrestlers, and may modulate biochemical response to training. PMID: 28482710
  27. Radiation-induced SOD2 overexpression via the chimeric C9BC promoter increased the radiosensitivity of HT-29 human colorectal cancer cells and concurrently protected normal CCD 841 CoN colorectal cells from radiation damage. PMID: 27999194
  28. research demonstrated that erysipelas infection predisposition and its clinical characteristics are affected by age, sex and SNPs found in SOD1, SOD2, and catalase genes; presence of SOD2 T2734 alleles was linked to erysipelas' predisposition; T and C alleles of SOD2 T2734C individually were linked to patients with bullous and erythematous erysipelas, respectively PMID: 28512644
  29. MnSOD plays an important integrative role in supporting cancer cell survival in circulation, metastasis, and doxorubicin resistance PMID: 27384484
  30. SOD2 rs4880 SNP likely contributes to asparaginase-induced hepatotoxicity in adult patients with acute lymphoblastic leukemia. PMID: 27019981
  31. Manganese superoxide dismutase mediates anoikis resistance and tumor metastasis in nasopharyngeal carcinoma cells. PMID: 27083052
  32. Preliminary neutron diffraction analysis of human manganese superoxide dismutase has been reported. PMID: 28368283
  33. Altogether, our results provide clinical evidence for the importance of SOD2 in tumor progression and mortality, and the close relationship of SOD2 and p53 in hepatocellular carcinoma. PMID: 27221200
  34. SOD2 and GPX1 can interact to affect cancer risk and progression indicated that it is the net accumulation of mitochondrial H2O2 (mtH2O2) resulting from of the balance between the activities SOD2 and anti-oxidants such as GPX1 that determines whether SOD2 prevents or promotes oncogenesis. Review. PMID: 28087256
  35. Low MnSOD expression is associated with Prostate Cancer. PMID: 28108513
  36. High MNSOD expression is associated with Thyroid Tumors. PMID: 26970173
  37. Findings suggest that the Ala16Val-MnSOD SNPs may contribute to hypercholesterolemia and higher GLU levels, increasing the risk to neurovascular events that may lead to stroke. PMID: 28552711
  38. Results suggest some pharmacogenetic effects of Val16Ala-SOD2 in hypercholesterolemia patients undergoing rosuvastatin treatment. PMID: 26882122
  39. he data demonstrated that linalool exhibited inhibitory effect on glioma cells through regulation of SIRT3-SOD2-ROS signaling PMID: 28567457
  40. WT-MnSOD protein conserves a destabilizing amino acid at position 146 as part of a strategy to favor metal ion binding. PMID: 28704037
  41. There is significant association between SOD rs4880 polymorphism and pulmonary arterial hypertension (PAH) susceptibility, and this polymorphism influenced PAH susceptibility by altering the expression of SOD2. PMID: 28272301
  42. It is suggested that the MnSOD A16V polymorphism may be associated with a risk of female infertility in northern Iran. PMID: 28040123
  43. Changes of Sig1R and SOD2 expression point to mitochondria as main organelle responsible for survival of tumor cells exposed to hypoxia or oxidative stress. Studied proteins are involved in intracellular response to stress related with different concentrations of oxygen. PMID: 27829319
  44. These results suggest SOD2 as a newly identified gene conferring susceptibility to leprosy. PMID: 27132285
  45. Results suggest that MnSOD Val(16)Ala single-nucleotide polymorphism may not contribute to lung cancer susceptibility. PMID: 23928928
  46. meta-analysis suggested that SOD2 C47T polymorphism is significantly associated with an increased risk of noise-induced hearing loss in the Chinese population. PMID: 27161188
  47. Study demonstrates that SOD2 rs4880, GPX1 rs1050450 and CAT rs1001179 are not associated with an increased susceptibility to epilepsy after neonatal hypoxic-ischemic encephalopathyor its drug resistance PMID: 28222320
  48. The antioxidative effects of the Mediterranean diet against breast cancer risk may be enhanced by the wild-type alleles of the MnSOD or CAT SNPs among Greek-Cypriot women. PMID: 26130326
  49. Lower SOD2 protein content in mononuclear cells Is associated with improved survival in chronic kidney disease patients undergoing hemodialysis therapy. PMID: 27630759
  50. we identify miR-146a as a potential tumor suppressor in patients with EOC. miR-146a downregulates the expression of SOD2 and enhances ROS generation, leading to increased apoptosis, inhibition of proliferation, and enhanced sensitivity to chemotherapy. PMID: 27131313
  51. The effects of hypericin and methoxyestradiol on the expression of SOD2 in MCF-7 cells and the sensitivity of these cells to photodynamic therapy are reported. PMID: 27923686
  52. Mn-SOD overexpression is a common event in colorectal cancer PMID: 26846100
  53. variants of GPx1 and MnSOD should not be considered as a risk factor of laryngeal squamous cell carcinoma. PMID: 27188866
  54. 47T>C (rs4880) genotypes associated with risk for alcoholic cirrhosis PMID: 26873981
  55. There were no significant between-group differences in the CC genotype distribution of the p.Ala16Val (C>T) polymorphism in the SOD2 gene. PMID: 27534583
  56. Results showed no statistically significant difference between the vitiligo and MnSOD Ala-9Val and GPx1 Pro198Leu gene polymorphisms in Turkish population. PMID: 27100222
  57. Authors identify miR-146a as a potential tumor suppressor in patients with EOC. miR-146a downregulates the expression of SOD2 and enhances ROS generation, leading to increased apoptosis, inhibition of proliferation, and enhanced sensitivity to chemotherapy. PMID: 27931283
  58. endogenous Alu RNA accumulation in hyperglycemic endothelial cells provokes endothelial oxidative stress and dysfunction by suppressing SOD2 and eNOS expression at both transcription and translation levels via NFkappaB signaling pathway. PMID: 26891959
  59. The MnSOD, GPX1 and CAT genotypes and allele frequencies of acute kidney injury patients did not differ significantly from those of healthy controls. PMID: 26787049
  60. These results suggest that the MnSOD Ala-9Val polymorphism may not influence smoking status in a Chinese male schizophrenia population. PMID: 26544677
  61. In this review, we will conglomerate the current aspects by which MnSOD can contribute to embryonic stem cells' and adult stem cells' functions and interpret the necessity of understanding MnSOD for further stem cell mediated applications PMID: 26899340
  62. patients with end-stage renal disease (ESRD) and those without chronic kidney disease (CKD) of Han Chinese origin, with SOD2 (Val16Ala), GPX1 (Pro197Leu), and PPAR-gamma (Pro12Ala, C161T) were genotyped. PMID: 26881045
  63. No correlation was observed between susceptibility to spontaneous abortion and MnSOD Val16Ala polymorphism. PMID: 25140979
  64. MnSOD emerges as a poor prognostic biomarker in p53 expressing glioblastoma PMID: 26616112
  65. Descriptive Statement Data show that caveolin-1 (Cav-1) expression in MCF7 breast cancer epithelial cells represses NF-E2-related factor 2 (Nrf2) and superoxide dismutase (MnSOD). PMID: 26543228
  66. Our results showed a lack of association of Pro198Leu GPx polymorphism to coronary heart disease risk and severity. However, they suggest that Ala16Val MnSOD polymorphism and decreased antioxidant defences are likely contributed to CHD risk in Tunisian men. Furthermore, the Val encoding MnSOD allele and decreased SOD activity were significantly correlated with CHD stenosis progression. PMID: 27067415
  67. By inhibiting mTOR and MnSOD, we confirmed that rapamycin functioned through the mTOR/MnSOD/reactive oxygen species (ROS) signaling pathway, and the existence of Akt governed the rapamycininduced asymmetric division PMID: 26707081
  68. miR-140-5p at least partly targets Dnmt1 and regulates SOD2 expression to inhibit proliferation and promote apoptosis and differentiation of HPASMCs in hypoxia PMID: 27021683
  69. The results indicate that common variants in MnSOD and eNOS increased the risk of T2DM in the Chinese Han population. PMID: 26505452
  70. SOD2 genetic variant associated with treatment-related ototoxicity in cisplatin-treated pediatric medulloblastoma PMID: 26400460
  71. MnSOD Val-9Ala gene polymorphism has a positive association with the risk for aggressive periodontitis. PMID: 26718428
  72. Although T allele of the SOD2 rs4880 SNP gene (rs4880-T) is not an independent risk for aMCI or AD, this allele increases the risk to aMCI patients carrying at least one APOEepsilon4 allele. PMID: 26696693
  73. Thus these findings suggest that the three variants of ADH1C, MnSOD and GSTM1 can be used to identify individuals who are at high risk to develop ALD and may be helpful in proper management of Indian alcoholics PMID: 26937962
  74. SOD2 single nucleotide polymorphisms and occurrence of osteoporosis in Asian Indians PMID: 26336112
  75. Data show that increased microRNA miR-301a expression is associated with decreased manganese superoxide dismutase (MnSOD) expression in pancreatic ductal adenocarcinoma (PDAC) specimens. PMID: 26384137
  76. The Mn-SOD expression was more frequently present in specimens with severe and moderate inflammation of gastric mucosa than in those with normal mucosa. PMID: 26765960
  77. Study shows that the SOD2 16C/T and CAT -21A/T alleles may serve as useful genetic susceptibility markers for migraine PMID: 25818327
  78. The Mn-SOD 47 C variant in combination with high tea consumption may provide protection against complications in type 2 diabetes mellitus. PMID: 26345792
  79. results suggest that the effect of resveratrol on human peripheral blood mononuclear cell activation is not universal and is dependent on the Ala16Val-SOD2 SNP. PMID: 25753816
  80. the ratios SOD2/catalase and SOD2/Gpx1 could be considered as potential markers during progression from tumor growth to metastasis PMID: 25866291
  81. The high expression of MnSOD indicates a slow tumor progression, suggesting a better prognosis and this may serve as an indicator to determine the biological behavior of esophageal cancer, and to provide the targeted therapy. PMID: 21143698
  82. These results suggest that melatonin exerts a hepatoprotective effect on mitochondrial-derived superoxide-stimulated autophagic cell death that is dependent on the SIRT3/SOD2 pathway. PMID: 26120888
  83. Germline genetic variation in the SOD2 gene might be a predictive biomarker of response to radiotherapy for prostate cancer. PMID: 25662905
  84. Data show that imatinib treatment of chronic myelogenous leukemia (CML) cell lines results in an approximately four-fold increase in the levels of manganese superoxide dismutase (MnSOD), without altering the levels of the corresponding transcript. PMID: 25039350
  85. Subepidermal SOD2 area in the distal leg was increased in the diabetic group vs the controls and was correlated with an increasing duration of diabetes PMID: 25933618
  86. we show that mitochondrial superoxide dismutase (Sod2) is highly expressed in ovarian clear cell carcinoma compared with other epithelial ovarian cancer subtypes PMID: 26359457
  87. inhibits the gastric carcinoma cell proliferation via induction of apoptosis PMID: 25889634
  88. c.47T>C polymorphism (SNP rs4880) in the manganese superoxide dismutase (SOD2) gene is a risk factor for primary angle closure glaucoma (PACG) in the Saudi population. PMID: 24070129
  89. LRIG1 can enhance chemosensitivity in glioblastoma by inhibition of BCL-2 and MnSOD PMID: 25449296
  90. MnSOD up-regulation sustains the Warburg effect via mitochondrial reactive oxygen species and AMPK-dependent signaling in cancer. PMID: 25651975
  91. we revealed that SOD2 was required to maintain monocyte differentiation into functional OCs and may become a potential target for regulating the efficiency of OTM in the future PMID: 25619900
  92. indicated that it was more common for MnSOD-positive, nuclear p65-positive, or high Bcl-2 mRNA tumors to have an unfavorable response to cisplatin-based chemotherapy than their counterparts PMID: 25499851
  93. SOD2-mediated antioxidant activity may restrict conversion of low CD44 expressing cells to high CD44 expressing cells at the early stages of epithelial-mesenchymal transition. PMID: 25659582
  94. Variations in CTSD and MnSOD showed no association with the development of Alzheimer's Disease, whereas the presence of the Ala224Val polymorphism in CTSD had a positive association with the development of AD PMID: 26351775
  95. miR-21 regulated SOD2, suggesting that miR-21 might mediate bystander responses through its regulation on SOD2. PMID: 26302379
  96. Decreased MnSOD activity may be associated with the risk of chronic schizophrenia in Chinese population, while MnSOD Ala-9Val polymorphism may not play a significant role in the development of Schizophrenia and Tardive dyskinesia . PMID: 26356721
  97. MnSOD Val-9Ala gene polymorphism is associated with gastric cancer. PMID: 25684475
  98. 593C>T GPx1 SNP alone and in synergy with 47C>T SOD2 SNP on the outcome of critically ill patients PMID: 25497738
  99. no other signs of combined effects, except for a combined effect of ECSOD Ala40Thr and MnSOD Val16Ala in non-HBV carriers, were observed for each combination of these four polymorphisms PMID: 25894370
  100. In transgenic mice with smooth muscle- specific deletion of SOD2, lower functional bladder capacity was seen. PMID: 25948732

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Involvement in disease Microvascular complications of diabetes 6 (MVCD6)
Subcellular Location Mitochondrion matrix
Protein Families Iron/manganese superoxide dismutase family
Database Links

HGNC: 11180

OMIM: 147460

KEGG: hsa:6648

STRING: 9606.ENSP00000356022

UniGene: Hs.487046

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