Human Syndecan-3(SDC3)ELISA Kit

Instructions
Code CSB-E14984h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name syndecan 3
Alternative Names N-syndecan ELISA Kit; Sdc3 ELISA Kit; SDC3_HUMAN ELISA Kit; SDCN ELISA Kit; SYND3 ELISA Kit; Syndecan neural type ELISA Kit; syndecan proteoglycan 3 ELISA Kit; Syndecan-3 ELISA Kit
Abbreviation SDC3
Uniprot No. O75056
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SDC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %80-99
1:2Average %92
Range %85-105
1:4Average %97
Range %90-103
1:8Average %93
Range %82-98
Recovery
The recovery of human SDC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9586-105
EDTA plasma (n=4)9789-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.814 2.753 2.784 2.616
15002.246 2.177 2.212 2.044
7501.345 1.286 1.316 1.148
3750.721 0.704 0.713 0.545
187.50.433 0.452 0.443 0.275
93.750.334 0.357 0.346 0.178
46.880.248 0.242 0.245 0.077
00.171 0.165 0.168
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
Gene References into Functions
  1. High PTN and N-syndecan expression was closely associated with metastasis and poor prognosis, suggesting that they may promote tumor progression and perineural invasion in the orthotopic mouse model of pancreatic cancer. PMID: 28638231
  2. Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells PMID: 25617766
  3. Data indicate that the extracellular and cytoplasmic domains of syndecans 1/2/3/4 are intrinsically disordered regions. PMID: 24956062
  4. frequently expressed in pancreatic cancer and associated with perineural invasion PMID: 24659893
  5. Syndecan-3 expression was not associated with disease progression and outcome in specific subtypes of mammary carcinoma. PMID: 23351331
  6. SDC3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
  7. Syndecan-3 may directly transduce Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFL) signals or serve as a coreceptor, presenting GFLs to the signaling receptor RET. PMID: 21200028
  8. These findings indicate that SDC3 polymorphisms could contribute to the link between female hyperandrogenism and obesity and suggest a novel potential role for SDC3 as a modulator of gonadal steroid function. PMID: 19820907
  9. Augmented synthesis and differential localization of heparan sulfate proteoglycans in Duchenne muscular dystrophy. PMID: 11968010
  10. Selective induction of CXCL8 binding site on endothelial syndecan-3 in rheumatoid arthritis(RA) synovium. This site may be involved in leukocyte trafficking into RA synovial tissue. PMID: 16052590
  11. The high expression of syndecan 3 and its localization to the smooth muscle bundles during normal labour, together with the significant reduction in prolonged labour, may indicate a role for this protein in the co-ordination of myometrial contractility. PMID: 16674815
  12. There are ethnic differences in the SDC3 polymorphisms, and the polymorphisms are strongly associated with obesity. PMID: 17018662
  13. Midkine, pleiotrophin (PTN), and their receptors syndecan-3 and receptor protein tyrosine phosphatase beta/zeta, were highly expressed in the striatum during developmen PMID: 17368428
  14. Syndecan-3 was identified as a major HIV-1 attachment receptor on DCs. PMID: 18040049
  15. syndecan TMD homodimerization and heterodimerization can be mediated by GxxxG motifs and modulated by sequence context PMID: 18093920
  16. pleiotrophin (PTN) and syndecan-2 and -3 as direct binding partners of Y-P30. PMID: 18599487
  17. found in 57.9% of pancreatic ductal carcinoma specimens PMID: 18716876
  18. Data report that the bFGF, FGFR1/2 and syndecan 1-4 expressions are altered in bladder tumours. PMID: 19822079

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Syndecan proteoglycan family
Tissue Specificity Expressed in the nervous system, the adrenal gland, and the spleen.
Database Links

HGNC: 10660

OMIM: 186357

KEGG: hsa:9672

STRING: 9606.ENSP00000344468

UniGene: Hs.158287

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