Human T-lymphocyte surface antigen Ly-9(LY9) ELISA kit

Instructions
Code CSB-EL013253HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lymphocyte antigen 9
Alternative Names CD229 ELISA Kit; Cell surface molecule Ly-9 ELISA Kit; Cell surface molecule Ly9 ELISA Kit; hly9 ELISA Kit; LY 9 ELISA Kit; Ly9 ELISA Kit; LY9_HUMAN ELISA Kit; Lymphocyte antigen 9 ELISA Kit; mLY9 ELISA Kit; Signaling lymphocytic activation molecule 3 ELISA Kit; SLAM family member 3 ELISA Kit; SLAMF3 ELISA Kit; T lymphocyte surface antigen Ly9 precursor ELISA Kit; T-lymphocyte surface antigen Ly-9 ELISA Kit
Abbreviation LY9
Uniprot No. Q9HBG7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LY9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %99
Range %94-105
1:2Average %97
Range %91-103
1:4Average %92
Range %89-99
1:8Average %98
Range %90-104
Recovery
The recovery of human LY9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9185-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20001.952 2.085 2.019 1.914
10001.539 1.562 1.551 1.446
5001.081 1.094 1.088 0.983
2500.697 0.689 0.693 0.588
1250.462 0.448 0.455 0.350
62.50.371 0.399 0.385 0.280
31.250.256 0.269 0.263 0.158
00.103 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A
Gene References into Functions
  1. Authors make a strong correlation between induced SLAMF3 overexpression and the specific loss of MRP-1 expression and its functionalities as a drugs resistance transporter. PMID: 27081035
  2. the results presented here suggest that the tumor suppressor potential of SLAMF3 occurs through activation of Retinoblastoma protein that represses PLK1. PMID: 26799423
  3. study showed CD229 is overexpressed on the malignant plasma cells of patients across all types of plasma cell dyscrasias including multiple myeloma; CD229 is also expressed on the surface of a fraction of cells carrying the phenotype of chemotherapy-resistant and myeloma-propagating cells within the patients' bone marrow PMID: 26001047
  4. the Val602 variant of the non-synonymous single nucleotide polymorphism (SNP) rs509749 in the SLAM family member CD229 (Ly9, SLAMF3) has a two-fold lower affinity compared with the SLE-associated Met602 variant for the small adaptor protein SAP PMID: 26221972
  5. CD229 Expression on Bone Marrow Plasma Cells from Patients with Multiple Myeloma and Monoclonal Gammopathies of Uncertain Significance. PMID: 26303094
  6. Results revealed that SLAMF3 plays a role during hepatitis C virus entry, likely by enhancing entry of viral particle within hepatocytes. PMID: 24927415
  7. SLAMF3 is an inhibitor of hepatocellular carcinoma cell proliferation and tumor progression. PMID: 24376606
  8. These results suggest a role for CD319 and CD229 in the systemic lupus erythematosus disease process. PMID: 23956418
  9. Data indicate that the dominance of the SLAMF3/SLAMF6 pathway in inducing IL-17A production can be attributed to an increased nuclear abundance and recruitment of RORgammat to the IL17A promoter. PMID: 22989874
  10. SLAMF3 and SLAMF6 T cell surface expression and IL-17 levels significantly correlate with disease activity in systemic lupus erythematosus patients PMID: 22184727
  11. CD229 is specifically over-expressed on myeloma cells including their clonogenic precursors and contributes to their malignant phenotype. PMID: 21606160
  12. Both mouse and human novel Ly9 genes mapped close to the CD229 gene in a region where other members of the CD150 family have also been mapped. PMID: 12242590
  13. CD229, but not other members of the CD150 family, directly bind Grb2. PMID: 15879090
  14. CD229 is a self-ligand, interacting through its N-terminal V-like domain which contains three amino acid residues critical for the homophilic binding interaction. PMID: 15905546
  15. A family-based association study in the United Kingdom and Canada identifies genetic variants in the LY9 promoter and coding region contributing to systemic lupus erythematosus susceptibility. PMID: 18216865

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Subcellular Location Membrane, Single-pass type I membrane protein, Cell membrane
Tissue Specificity Increased surface expression on T-cells of systemic lupus erythematosus (SLE) patients.
Database Links

HGNC: 6730

OMIM: 600684

KEGG: hsa:4063

STRING: 9606.ENSP00000357020

UniGene: Hs.403857

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