Human TNFAIP3-interacting protein 1(TNIP1) ELISA kit

Instructions
Code CSB-EL023999HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name TNFAIP3 interacting protein 1
Alternative Names ABIN 1 ELISA Kit; HIV-1 Nef-interacting protein ELISA Kit; hVAN ELISA Kit; KIAA0113 ELISA Kit; Naf1 ELISA Kit; Nef-associated factor 1 ELISA Kit; Nip40-1 ELISA Kit; TNFAIP3 interacting protein 1 ELISA Kit; TNFAIP3-interacting protein 1 ELISA Kit; TNIP-1 ELISA Kit; Tnip1 ELISA Kit; TNIP1_HUMAN ELISA Kit; VAN ELISA Kit; Virion-associated nuclear shuttling protein ELISA Kit
Abbreviation TNIP1
Uniprot No. Q15025
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNIP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-91
1:2 Average % 97
Range % 91-103
1:4 Average % 97
Range % 91-102
1:8 Average % 88
Range % 83-92
Recovery
The recovery of human TNIP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 91-103
EDTA plasma (n=4) 87 83-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.625 2.726 2.676 2.569
750 2.024 2.148 2.086 1.979
375 1.378 1.483 1.431 1.324
187.5 0.902 0.911 0.907 0.800
94 0.494 0.502 0.498 0.391
47 0.256 0.265 0.261 0.154
23.5 0.212 0.202 0.207 0.100
0 0.106 0.108 0.107  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.
Gene References into Functions
  1. this review discusses the role of TNIP1 in autoimmune diseases as a regulator of Toll-like receptor signaling PMID: 30402506
  2. Results showed that ABIN1 acted as a modulator to down-regulate HDAC1 ubiquitination via three different sites, thereby stabilizing HDAC1 by inhibiting its lysosomal and proteasomal degradation. PMID: 29058807
  3. Results provide evidence that TNIP1 SNP (rs7708392) might be associated with the development of lupus nephritis in Egyptian SLE patients. PMID: 29589214
  4. cell viability and apoptosis in the hepatic cell line L02 were detected after hypoxia/reoxygenation (H/R) treatment, and MTT assay showed that cell viability was inhibited after H/R treatment, but reversed after TNIP1 transfection PMID: 29709475
  5. association of TNIP1 was stronger in AIH without HLA-DRB1*04:05 allele (P = 0.0063, Q = 0.0127, OR 1.48, 95% CI: 1.12-1.96), though the association was not detected in AIH with DRB1*04:05 PMID: 29559739
  6. Naf1 contributes to the maintenance of HIV-1 latency by inhibiting long terminal repeat driven HIV-1 gene transcription in a nuclear factor kappa B-dependent manner. PMID: 27795436
  7. the risk allele for the TNIP1 rs7708392 polymorphism was associated with systemic lupus erythematosus susceptibility. PMID: 29783072
  8. C/EBP beta LAP isoform expression was increased and LIP/TNFAIP3/TNIP1 expression was decreased in systemic lupus erythematosus (SLE) patients. LAP expression was positively correlated with SLE disease activity; TNFAIP3 and TNIP1 expression was negatively correlated with SLE disease activity. PMID: 27659348
  9. genetic variation in TNIP1 gene is associated with gastric carcinoma PMID: 27250029
  10. An association of the GPX3-TNIP1 locus with ALS is identified using cross-ethnic meta-analyses. [Meta-Analysis] PMID: 28931804
  11. These studies define a novel role for ABIN1 dysfunction and NF-kappaB in mediating glomerulonephritis through proinflammatory activation of podocytes. PMID: 28935578
  12. IM could upregulate A20 protein to inhibit the activation of NF-kappaB pathway in Jurkat T cells, which was independent of the ABIN1 protein. PMID: 28502291
  13. LILRB1 ligation during monocyte differentiation to dendritic cells in vitro increases ABIN1 expression. Increased ABIN1 expression occurs in "immunosuppressive" monocytes of patients with non-Hodgkin lymphoma ex vivo. ABIN1 siRNA allows dendritic cells and immunosuppressive monocytes to respond to stimulation by allowing NF-kappaB translocation to the nucleus, more phagocytosis, cytokine secretion, and T-cell stimulation. PMID: 27129285
  14. These findings show ABIN1 as an intrinsic suppressor of HIV-1 mRNA transcription by regulating the ubiquitination of Tat. PMID: 28193275
  15. Polymorphisms in TNIP1 are associated with GPP in Chinese Han population. However, no association with PPP was found. These findings suggest that TNIP1 might be a susceptibility gene for GPP. PMID: 27364786
  16. The findings of this study suggested that TNIP1 is a new potential predisposing gene to spina bifida (SB) and its pathway needs to be investigated in human NTD in order to confirm its role and to plan appropriate counseling to families PMID: 27125519
  17. Data suggest that reduced TNFalpha-induced protein 3-interacting protein 1 (TNIP1) expression may have a role in the pathogenesis of myasthenia gravis (MG)for patients with thymoma. PMID: 27577206
  18. HIV-1 depends on host-cell-encoded factors to complete its life cycle; data suggest that nucleus-located NAF1/TNIP1 promotes nuclear export of un-spliced HIV-1 gag mRNA; association between NAF1/TNIP1 and CRM1 is required for this function. PMID: 26733199
  19. Hepatitis C virus antigen stimulation up-regulates A20/A20-binding inhibitor of NF-kappaB binding protein expression, which consequently contributes to inefficient M1 macrophage polarization. PMID: 26382585
  20. Data suggest that tumour necrosis factor alpha-induced protein 3 (TNFAIP3) and TNFAIP3-interacting protein 1 (TNIP1) single nucleotide polymorphisms (SNPs) confer increased risk of psoriasis in ethnic South Indian Tamils. PMID: 26738398
  21. Since TNIP1 may facilitate the function of TNFAIP3 in the negative feedback regulation of NF-kappaB activation [5], the corresponding transcript levels of TNIP1 in psoriasis vul- garis were investigated in this study. PMID: 25631139
  22. The aim of this study was to investigate whether the HCP5, TNIP1, TNFAIP3, SPATA2 and COG6 genes were genetic risk factors for psoriasis in Chinese population. PMID: 25264125
  23. Single-nucleotide polymorphisms rs7708392 and rs495881 in TNIP1 were significantly associated with Lupus Nephritis in individuals with European or African ancestry. PMID: 25034154
  24. HSPA6 regulation by TNIP1 occurs in promoter regions lacking binding sites for known TNIP1-repressed transcription factors PMID: 25447897
  25. Report role of TNIP1 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
  26. TNIP1 polymorphism may be a risk factor for VKH syndrome in Han Chinese PMID: 24788730
  27. Analysis of SNP in the TNIP1 gene and expression of this gene in peripheral blood lymphocytes indicated these SNP were associated with the occurrence of systemic lupus erythematosus in Han Chinese patients. PMID: 23858047
  28. We conclude that polymorphisms in TNIP1 are associated with antibody-positive primary Sjogren's syndrome. PMID: 23944604
  29. variants in the TNIP1 gene are associated with the risk for lupus nephritis and could be mechanistically involved in disease development via aberrant regulation of NF-kappaB and mitogen-activated protein kinase activity. PMID: 23970121
  30. TNIP1/ANXA6 and CSMD1 variants interacting with cigarette smoking and alcohol intake affect risk of psoriasis. PMID: 23541940
  31. Data indicate significant multiplicative interaction observed between NFKB1 rs28362491 and TNIP1 rs3792783 in systemic lupus erythematosus. PMID: 23911423
  32. Analysis of the TNIP1 promoter reveals multiple transcription start sites in its GC-rich proximal regions along with two transcriptionally active specificity protein (SP) sites, responsive to both Sp1 and Sp3. PMID: 23464785
  33. These data confirmed the influence of TNIP1 on an increased susceptibility to systemic sclerosis and reinforced this locus as a common autoimmunity risk factor. PMID: 22896740
  34. This study demonistrated that (151) Pro-->Ala change in TNIP1 association to myasthenia gravis. PMID: 23055271
  35. Our results confirm the association signals between systemic lupus erythematosus and TNIP1 variants in multiple populations and provide new insight into the mechanism by which TNIP1 variants may contribute to SLE pathogenesis. PMID: 22833143
  36. Data indicate that TNFAIP3, ETS1 and TNIP1 are probably common susceptibility genes for systemic lupus erythematosus (SLE) in Chinese populations, and they may contribute to the pathogenesis of multiple SLE subphenotypes. PMID: 22087647
  37. we integrate the current knowledge of TNIP1s functions with the diseases in which it has been associated to potentially elucidate the role this regulator has in promoting or alleviating these inflammatory diseases[review] PMID: 22542476
  38. The TNIP1 distal promoter, approximately 4600 nucleotides upstream of a frequently utilized transcription start site region, contains PPAR-gamma and NF-kappaB sites that bind the respective transcription factors in vivo. PMID: 22001530
  39. recombinant transgene facilitates the transcription of pro-inflammatory cytokines in fibroblast-like synoviocytes in rheumatoid arthritis PMID: 22093807
  40. A markedly reduced expression of the TNIP1 gene and also its protein product were observed both in lesional skin tissue and in cultured dermal fibroblasts from SSc(Systemic sclerosis) patients. PMID: 21750679
  41. The requirements for TNIP1-peroxisome proliferator activated receptor (PPAR) interaction are characteristic for coactivators; however, TNIP1 partially decreases PPAR activity. PMID: 21967852
  42. The varying levels and distribution of TNIP1 in normal and disease state tissues could be expected to affect processes in which TNIP1 is involved PMID: 22147607
  43. The researchers found a genotype association of SNP rs1808593 NOS3 polymorphisms and HIE. PMID: 21674837
  44. ABIN1 requires its ubiquitin binding domain and cooperates with TAX1BP1 and A20 to restrict antiviral signaling. PMID: 21885437
  45. The IL23A and TNIP1 genes showed convincing evidence for association SNPs mapping to previously reported psoriasis loci show evidence for association to PSA. PMID: 21623003
  46. the interaction of ABIN1 with polyubiquitin is required to limit the activation of TLR-MyD88 pathways and prevent autoimmunity PMID: 21606507
  47. A20, ABIN-1/2, and CARD11 mutations have prognostic value in gastrointestinal diffuse large B-cell lymphoma PMID: 21266526
  48. Association of TNIP1 with systemic lupus erythematosus was confirmed in a Japanese population PMID: 20849588
  49. Significant associations were found for the single nucleotide polymorphism rs10036748 of TNIP1 with photosensitivity and vasculitis in patients with systemic lupus erythematosus PMID: 20516000
  50. A new ERK2 binding protein, Naf1, attenuates the EGF/ERK2 nuclear signaling. PMID: 12220502
  51. TNF-alpha induces Naf1 gene expression in synoviocytes. PMID: 12965196
  52. alternative splicing and role implicated in interaction with HIV-1 PMID: 14526201
  53. NAF1 is required for accumulation of human box H/ACA, snoRNPs, scaRNPs, and telomerase. PMID: 16601202
  54. ABIN-1 physically links A20 to NEMO/IKKgamma and facilitates A20-mediated de-ubiquitination of NEMO/IKKgamma, thus resulting in inhibition of NF-kappaB PMID: 16684768
  55. Novel splice variants of the Naf1 gene were identified. PMID: 17016622
  56. NAF1 associates with mature H/ACA RNA PMID: 17135485
  57. an important role for ubiquitin binding in the negative regulation of NF-kappaB signaling by ABINs and identify UBAN as a novel UBD. PMID: 18212736
  58. role for an ABIN-sensitive non-classical NF-kappaB signalling pathway in the proliferation of EGFR-overexpressing tumour cells. PMID: 18622428
  59. Expanded catalog of genetic loci implicated in psoriasis susceptibility. PMID: 19169254
  60. The shRNA barcode screening technique identified TNIP1 as being involved in p53 regulation. PMID: 19277210
  61. This review defines ABIN-1 based on three different parameters: ability to bind A20; ability to inhibit NF-kappaB activation upon overexpression; the presence of specific short amino acid regions of strong homology, designated ABIN homology domains. PMID: 19464428
  62. the effects of ABIN-1 on p105 processing and of p105 on stabilizing ABIN-1 act to potentiate the NF-kappaB inhibitory activity of ABIN-1. PMID: 19695220
  63. TNIP1 interacts with liganded RARalpha and RARgamma but acts as a corepressor of their activity. PMID: 19732752
  64. identified five new systemic lupus erythematosus susceptibility loci (P < 5 x 10(-8)): TNIP1 (odds ratio (OR) = 1.27), PRDM1 (OR = 1.20), JAZF1 (OR = 1.20), UHRF1BP1 (OR = 1.17) and IL10 (OR = 1.19). PMID: 19838195

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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. In peripheral blood mononucleocytes, isoform 4 is mainly expressed and isoform 1 and isoform 7 are almost not expressed. Expr
Database Links

HGNC: 16903

OMIM: 607714

KEGG: hsa:10318

STRING: 9606.ENSP00000317891

UniGene: Hs.355141

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