Human Tenomodulin(TNMD) ELISA kit

Instructions
Code CSB-EL024007HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tenomodulin
Alternative Names BRICD4 ELISA Kit; BRICHOS domain containing 4 ELISA Kit; CHM1 LIKE ELISA Kit; ChM1L ELISA Kit; Chondromodulin I like ELISA Kit; Chondromodulin I like protein ELISA Kit; Chondromodulin-1-like protein ELISA Kit; Chondromodulin-I-like protein ELISA Kit; hChM1L ELISA Kit; hTeM ELISA Kit; Myodulin ELISA Kit; TeM ELISA Kit; Tendin ELISA Kit; Tenomodulin ELISA Kit; Tenomodulin protein ELISA Kit; Tnmd ELISA Kit; TNMD_HUMAN ELISA Kit; UNQ771/PRO1565 ELISA Kit
Abbreviation TNMD
Uniprot No. Q9H2S6
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNMD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-97
1:2 Average % 85
Range % 80-91
1:4 Average % 94
Range % 89-98
1:8 Average % 86
Range % 81-92
Recovery
The recovery of human TNMD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 99-108
EDTA plasma (n=4) 101 98-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.205 2.345 2.275 2.144
1000 1.745 1.887 1.816 1.685
500 1.219 1.245 1.232 1.101
250 0.745 0.756 0.751 0.620
125 0.463 0.457 0.460 0.329
62.5 0.317 0.328 0.323 0.192
31.25 0.225 0.229 0.227 0.096
0 0.130 0.132 0.131  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Q&A and Customer Reviews

 Q&A
Q:

in your instruction page6 -page7 "Dilute cell with 1xPBS (pH7.2-7.4), until cell concentration reached 100
million/ml."
What means '100 million/ml' ? How??

A:
Thanks for your inquiry!the mentioned concentration can be obtained by analysis of cell count .There is a recommanded culture concentration prior to implantation.And cells is with a Proliferative period and requires proper time.All these are available in the corresponding instructions of the cells.If any further questions,just let me know.

Target Data

Function May be an angiogenesis inhibitor.
Gene References into Functions
  1. our study demonstrates that Tnmd is required for proper tendon tissue adaptation to endurance running and aids in better understanding of the structural-functional relationships of tendon tissues. PMID: 28566251
  2. TNMD is upregulated in adipose tissue of insulin-resistant versus insulin-sensitive individuals. TNMD expression increases in human preadipocytes during differentiation, whereas silencing TNMD blocks adipogenesis. PMID: 26880110
  3. results suggest that Tnmd acts on the maturation or maintenance of the PDL by positively regulating cell adhesion via its BRICHOS domain PMID: 23593173
  4. Report differential expression and cellular localization of novel isoforms of the tendon biomarker tenomodulin and suggest possible roles in tenocyte proliferation and tendon injury. PMID: 22700804
  5. According to these results the sequence variation of TNMD is not associated with alzheimer disease, but might modify the effect of APOE epsilon4-allele in women. PMID: 19524323
  6. Polymorphisms are associated with adiposity and also with glucose metabolism and conversion from glucose intolerance to type 2 diabetes. PMID: 17495183
  7. Tenomodulin is expressed abundantly in the elastin-rich subendothelial outer layer of the normal chordae tendineae cordis. PMID: 18838562
  8. TNMD could be a novel candidate gene for age-related macular degeneration. PMID: 19381347
  9. Human adipose tissue TNMD gene expression is highly affected by obesity, adipose tissue location, and weight loss, indicating that TNMD may play a role in adipose tissue function. PMID: 19602561

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Subcellular Location Isoform 1: Membrane, Single-pass type II membrane protein, Nucleus envelope, SUBCELLULAR LOCATION: Isoform 2: Membrane, Single-pass type II membrane protein, Nucleus envelope, SUBCELLULAR LOCATION: Isoform 3: Cytoplasm
Protein Families Chondromodulin-1 family
Tissue Specificity Highly expressed in hypovascular connective tissues such as tendons. Has also strong expression in adipose tissue.
Database Links

HGNC: 17757

OMIM: 300459

KEGG: hsa:64102

STRING: 9606.ENSP00000362122

UniGene: Hs.132957

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