Human Transcription Factor/Activator Protein 1 (AP-1) ELISA kit

Code CSB-E13403h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05412
Lead Time 3-5 working days
Abbreviation JUN
Protein Biological Process 1 Transcription/Transcription regulation
Target Name jun oncogene
Alias AP-1, AP1, c-Jun, Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
HGNC 20184
RGD 1308917
MGI 1915057
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 107    
Range % 103-110    
1:2 Average % 94    
Range % 90-98    
1:4 Average % 102    
Range % 97-106    
1:8 Average % 89    
Range % 85-96    
Recovery
The recovery of human AP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 100 95-105    
EDTA plasma (n=4) 86 83-89    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
3000 2.755 2.805 2.780 2.616    
1500 2.374 2.231 2.303 2.139    
750 1.545 1.505 1.525 1.361    
375 0.897 0.868 0.883 0.719    
187.5 0.477 0.498 0.488 0.324    
94 0.324 0.331 0.328 0.164    
47 0.261 0.263 0.262 0.098    
0 0.166 0.162 0.164      
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Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells
Subcellular Location Nucleus
Protein Families BZIP family, Jun subfamily
Database Links

HGNC: 6204

OMIM: 165160

KEGG: hsa:3725

STRING: 9606.ENSP00000360266

UniGene: Hs.696684

Pathway cAMP signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
MAPK signaling pathway
Wnt signaling pathway
Focal adhesion
TNF signaling pathway
Apoptosis
B cell receptor signaling pathway
IL-17 signaling pathway
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Neurotrophin signaling pathway

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