Human Transcription factor 20(TCF20) ELISA kit

Unavailable
Code CSB-EL023296HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9UGU0
Lead Time 5-7 working days
Abbreviation TCF20
Protein Biological Process 1 Transcription/Transcription regulation
Target Name transcription factor 20 (AR1)
Alias RP4-669P10.13, AR1, KIAA0292, SPBP, SPRE-binding protein|nuclear factor SPBP|stromelysin 1 PDGF-responsive element-binding protein|stromelysin-1 platelet-derived growth factor-responsive element bin
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein binds a platelet-derived growth factor-responsive element in the matrix metalloproteinase 3 (stromelysin 1) promoter. The protein localizes to the nucleus and displays DNA-binding and transactivation activities. It is thought to be a transcriptional coactivator, enhancing the activity of transcription factors such as JUN and SP1. Alternative splicing results in two transcript variants encoding different isoforms.
HGNC 11631
RGD 1594486
MGI 108399
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TCF20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 84
Range % 80-88
1:4 Average % 90
Range % 85-94
1:8 Average % 93
Range % 88-97
Recovery
The recovery of human TCF20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 103 99-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 1.990 2.059 2.025 1.902
500 1.623 1.698 1.661 1.538
250 1.325 1.372 1.349 1.226
125 1.012 1.065 1.039 0.916
62.5 0.652 0.614 0.633 0.510
31.25 0.431 0.433 0.432 0.309
15.6 0.246 0.257 0.252 0.129
0 0.125 0.120 0.123  
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Function Transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator.
Subcellular Location Nucleus
Tissue Specificity Expressed in most tissues, except in ovary and prostate. Isoform 1 is exclusively expressed in brain, heart and testis, and this form predominates in liver and kidney. Isoform 2 predominates in lung.
Database Links

HGNC: 11631

OMIM: 603107

KEGG: hsa:6942

STRING: 9606.ENSP00000352463

UniGene: Hs.475018

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