Human Transcription factor SOX-2(SOX2) ELISA kit

Instructions
Code CSB-EL022426HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name SRY (sex determining region Y)-box 2
Alternative Names ANOP3, MCOPS3, MGC2413, SRY-related HMG-box gene 2|sex-determining region Y-box 2|transcription factor SOX2
Abbreviation SOX2
Uniprot No. P48431
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-97
1:2Average %95
Range %90-102
1:4Average %93
Range %85-99
1:8Average %94
Range %83-98
Recovery
The recovery of human SOX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9287-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.031 2.098 2.065 1.948
51.449 1.500 1.475 1.358
2.50.938 0.944 0.941 0.824
1.250.671 0.682 0.677 0.560
0.6250.405 0.394 0.400 0.283
0.3120.249 0.242 0.246 0.129
0.1560.167 0.173 0.170 0.053
00.115 0.119 0.117
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Involvement in disease Microphthalmia, syndromic, 3 (MCOPS3)
Subcellular Location Nucleus
Database Links

HGNC: 11195

OMIM: 184429

KEGG: hsa:6657

STRING: 9606.ENSP00000323588

UniGene: Hs.518438

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