Human Transcription factor SOX-2(SOX2) ELISA kit

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Code CSB-EL022426HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P48431
Lead Time 5-7 working days
Abbreviation SOX2
Protein Biological Process 1 Transcription/Transcription regulation
Target Name SRY (sex determining region Y)-box 2
Alias ANOP3, MCOPS3, MGC2413, SRY-related HMG-box gene 2|sex-determining region Y-box 2|transcription factor SOX2
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
HGNC 11195
RGD 1565646
MGI 98364
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-97
1:2Average %95
Range %90-102
1:4Average %93
Range %85-99
1:8Average %94
Range %83-98
Recovery
The recovery of human SOX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9287-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.031 2.098 2.065 1.948
51.449 1.500 1.475 1.358
2.50.938 0.944 0.941 0.824
1.250.671 0.682 0.677 0.560
0.6250.405 0.394 0.400 0.283
0.3120.249 0.242 0.246 0.129
0.1560.167 0.173 0.170 0.053
00.115 0.119 0.117
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Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Involvement in disease Microphthalmia, syndromic, 3 (MCOPS3)
Subcellular Location Nucleus
Database Links

HGNC: 11195

OMIM: 184429

KEGG: hsa:6657

STRING: 9606.ENSP00000323588

UniGene: Hs.518438

Pathway Hippo signaling pathway
Signaling pathways regulating pluripotency of stem cells

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