Human Transcriptional enhancer factor TEF-1(TEAD1) ELISA kit

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Code CSB-EL023363HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P28347
Lead Time 7-14 working days
Abbreviation TEAD1
Target Name TEA domain family member 1 (SV40 transcriptional enhancer factor)
Alias AA, REF1, TCF13, TEF-1, SV40 transcriptional enhancer factor|TEA domain family member 1|atrophia areata, peripapillary chorioretinal degeneration|transcription factor 13|transcriptional enhancer fac
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 37.5 pg/mL-2400 pg/mL
Sensitivity 9.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
HGNC 11714
RGD 1549742
MGI 101876
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TEAD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 88-96
1:2 Average % 102
Range % 95-106
1:4 Average % 94
Range % 86-98
1:8 Average % 100
Range % 94-105
Recovery
The recovery of human TEAD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 82-93
EDTA plasma (n=4) 95 87-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2400 2.317 2.271 2.294 2.144
1200 1.541 1.518 1.530 1.380
600 0.805 0.811 0.808 0.658
300 0.467 0.454 0.461 0.311
150 0.307 0.292 0.300 0.150
75 0.246 0.231 0.239 0.089
37.5 0.191 0.184 0.188 0.038
0 0.153 0.147 0.150  
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Function Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
Involvement in disease Sveinsson chorioretinal atrophy (SCRA)
Subcellular Location Nucleus
Tissue Specificity Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Database Links

HGNC: 11714

OMIM: 108985

KEGG: hsa:7003

STRING: 9606.ENSP00000354588

UniGene: Hs.655331

Pathway Hippo signaling pathway

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