Human Transcriptional repressor protein YY1(YY1) ELISA kit

Code CSB-EL026297HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P25490
Lead Time 5-7 working days
Abbreviation YY1
Protein Biological Process 1 DNA damage/DNA repair
Target Name YY1 transcription factor
Alias DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1, INO80 complex subunit S|Yin and Yang 1 protein
Species Homo sapiens (Human)
Protein Biological Process 3 DNA damage
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
HGNC 10480
RGD 1307077
MGI 1929059
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human YY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 88-98
1:2 Average % 96
Range % 90-102
1:4 Average % 99
Range % 96-103
1:8 Average % 102
Range % 96-105
Recovery
The recovery of human YY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 89-97
EDTA plasma (n=4) 98 95-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected CV
10 2.341 2.360 2.351 2.180 0.57%
5 2.205 2.212 2.209 2.038 0.22%
2.5 1.947 1.987 1.967 1.796 1.44%
1.25 1.379 1.345 1.362 1.191 1.77%
0.625 0.747 0.802 0.775 0.604 5.02%
0.312 0.495 0.521 0.508 0.337 3.62%
0.156 0.325 0.332 0.329 0.158 1.50%
0 0.169 0.172 0.171   1.24%
References
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Function Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression
Involvement in disease Gabriele-de Vries syndrome (GADEVS)
Subcellular Location Nucleus matrix
Protein Families YY transcription factor family
Database Links

HGNC: 12856

OMIM: 600013

KEGG: hsa:7528

STRING: 9606.ENSP00000262238

UniGene: Hs.388927

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