Human Trichohyalin(TCHH) ELISA kit

Unavailable
Code CSB-EL023309HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q07283
Lead Time 5-7 working days
Abbreviation TCHH
Protein Biological Process 1 Developmental Protein
Target Name trichohyalin
Alias RP11-107M16.3, MGC157889, MGC157890, THH, THL, TRHY,
Species Homo sapiens (Human)
Protein Biological Process 3 Keratinization
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 11791
RGD 1309635
MGI 2177944
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TCHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 85-100
1:2 Average % 107
Range % 96-111
1:4 Average % 99
Range % 91-103
1:8 Average % 91
Range % 83-95
Recovery
The recovery of human TCHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 83-93
EDTA plasma (n=4) 93 87-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 2.070 2.041 2.056 1.939
600 1.313 1.286 1.300 1.183
300 0.846 0.825 0.836 0.719
150 0.568 0.550 0.559 0.442
75 0.351 0.343 0.347 0.230
37.5 0.244 0.232 0.238 0.121
18.75 0.166 0.161 0.164 0.047
0 0.118 0.116 0.117  
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Function Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
Involvement in disease Uncombable hair syndrome 3 (UHS3)
Protein Families S100-fused protein family
Tissue Specificity Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium.
Database Links

HGNC: 11791

OMIM: 190370

KEGG: hsa:7062

STRING: 9606.ENSP00000357794

UniGene: Hs.432416

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