Human Troponin C, skeletal muscle(TNNC2) ELISA kit

Instructions
Code CSB-EL024010HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name troponin C type 2 (fast)
Alternative Names TNNC2 ELISA Kit; Troponin C ELISA Kit; skeletal muscle ELISA Kit
Abbreviation TNNC2
Uniprot No. P02585
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 39.07 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNNC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 88-103
1:2 Average % 94
Range % 87-99
1:4 Average % 92
Range % 85-97
1:8 Average % 90
Range % 85-94
Recovery
The recovery of human TNNC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 84-97
EDTA plasma (n=4) 99 91-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.033 2.135 2.084 1.983
1250 1.458 1.423 1.441 1.340
625 0.943 0.974 0.959 0.858
312.5 0.633 0.642 0.638 0.537
156.25 0.313 0.325 0.319 0.218
78.13 0.238 0.226 0.232 0.131
39.07 0.162 0.158 0.160 0.059
0 0.102 0.100 0.101  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components
Protein Families Troponin C family
Database Links

HGNC: 11944

OMIM: 191039

KEGG: hsa:7125

STRING: 9606.ENSP00000361636

UniGene: Hs.182421

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