Human Zinc finger protein 644(ZNF644) ELISA kit

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Code CSB-EL026914HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9H582
Lead Time 5-7 working days
Abbreviation ZNF644
Protein Biological Process 1 Transcription/Transcription regulation
Target Name zinc finger protein 644
Alias BM-005, KIAA1221, MGC60165, MGC70410, Zep-2, OTTHUMP00000011764|zinc finger motif enhancer binding protein 2|zinc finger protein 644, isoform 2
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 29222
RGD 1306805
MGI 1277212
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ZNF644 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %83-90
1:2Average %104
Range %100-107
1:4Average %87
Range %83-92
1:8Average %86
Range %83-89
Recovery
The recovery of human ZNF644 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10097-103
EDTA plasma (n=4)8985-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.181 2.198 2.190 2.006
2001.744 1.704 1.724 1.540
1001.316 1.335 1.326 1.142
500.911 0.926 0.919 0.735
250.582 0.547 0.565 0.381
12.50.421 0.432 0.427 0.243
6.250.297 0.287 0.292 0.108
00.183 0.185 0.184
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Function May be involved in transcriptional regulation.
Involvement in disease Myopia 21, autosomal dominant (MYP21)
Subcellular Location Nucleus
Protein Families Krueppel C2H2-type zinc-finger protein family
Tissue Specificity Expressed in liver, placenta, retina and retinal pigment epithelium.
Database Links

HGNC: 29222

OMIM: 614159

KEGG: hsa:84146

STRING: 9606.ENSP00000337008

UniGene: Hs.173001

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