Human amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

Code CSB-E08299h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name amyloid beta peptide 1-40,Aβ1-40
Abbreviation Aβ1-40
Species Homo sapiens (Human)
Sample Types serum, tissue homogenates, cerebrospinal fluid (CSF)
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-92  
1:2 Average % 99  
Range % 91-105  
1:4 Average % 95  
Range % 92-98  
1:8 Average % 103  
Range % 96-110  
The recovery of human Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 89-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
8000 2.846 2.687 2.767 2.610  
4000 2.237 2.089 2.163 2.006  
2000 1.679 1.612 1.646 1.489  
1000 1.246 1.211 1.229 1.072  
500 0.736 0.697 0.717 0.560  
250 0.512 0.498 0.505 0.348  
125 0.303 0.296 0.300 0.143  
0 0.160 0.154 0.157    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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We are interested in this kit.Could you let me know the information about the following ?
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

Thanks for your inquiry.
Capture antibody:mouse monoclonal antibody;Detection antibody:goat polyclonal antibody.


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