Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Code CSB-E10684h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Aβ1-42
Target Name amyloid beta peptide 1-42,Aβ1-42 96T
Alias Amyloid beta 42(ABeta 42)
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 97  
Range % 92-110  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.302 2.379 2.341 2.244  
10 1.762 1.781 1.772 1.675  
5 1.124 1.189 1.157 1.060  
2.5 0.653 0.679 0.666 0.569  
1.25 0.375 0.398 0.387 0.290  
0.625 0.251 0.259 0.255 0.158  
0.312 0.151 0.153 0.152 0.055  
0 0.095 0.098 0.097    
References
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Q&A and Customer Reviews

Q&A
Q:

We are interested in following item.
#CSB-E10684h
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

A:
Thanks for your inquiry.Regarding the mentioned kit,we would like to inform the following information:
(Note:Capture:capture antibody coated on the plate;Detection: biotin-conjugated antibody)
#CSB-E10684h,Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
Customer Reviews
  • Sample type: Brain tissues
    Sample species: Human
    Review: the discrimination index in the object recognition test. After the MWM test and the object recognition test, animals were sacrificed and the brain tissues were collected. Aβ42 levels in the cortex (K) and hippocampus (L) were detected by ELISA. # p < 0.05, ###p < 0.001 vs. Wt group. * p < 0.05, **p < 0.01, ***p < 0.001 vs. APP/PS1 group.
    PMID: 30128052

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