Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

Instructions
Code CSB-E04493h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E04493h is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of human angiotensin II (ANG-II) in biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction, the ANG-II levels can be calculated according to the standard curve.

This Human ANG-II ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 10%, and lot-to-lot consistency.

ANG-II is a product of the renin-angiotensin-aldosterone system (RAAS). ANG-II exerts its strong vasopressor activity through vasoconstriction and elevated aldosterone release. The catalysis of ANG-II by ACE2 converts into angiotensin (1–7). Wolfgang Miesbach showed that SARS-CoV-2 uses the ACE2 receptor to enter the target cells, resulting in activation of the RAAS. After downregulating the ACE2, the vasoconstrictor ANG-II is increasingly produced and its counter regulating molecules angiotensin (1–7) reduced. ANG-II increases thrombin formation and impairs fibrinolysis. Elevated levels were strongly associated with viral load and lung injury in patients with severe COVID-19. Ang II contributes to endothelial dysfunction, the development of arteriosclerosis, and microvascular thrombosis. Ang II infusion leads to abnormal inflammatory and thrombotic reactions in the microcirculation. In hypertensive rats, Ang II accelerates arterial thrombosis of the carotid artery via the AT1 receptor.

Target Name angiotensin Ⅱ
Abbreviation ANG-Ⅱ
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANG-II antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled ANG-II antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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