The product CSB-E04493h is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of human angiotensin II (ANG-II) in biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction, the ANG-II levels can be calculated according to the standard curve.
This Human ANG-II ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 10%, and lot-to-lot consistency.
ANG-II is a product of the renin-angiotensin-aldosterone system (RAAS). ANG-II exerts its strong vasopressor activity through vasoconstriction and elevated aldosterone release. The catalysis of ANG-II by ACE2 converts into angiotensin (1–7). Wolfgang Miesbach showed that SARS-CoV-2 uses the ACE2 receptor to enter the target cells, resulting in activation of the RAAS. After downregulating the ACE2, the vasoconstrictor ANG-II is increasingly produced and its counter regulating molecules angiotensin (1–7) reduced. ANG-II increases thrombin formation and impairs fibrinolysis. Elevated levels were strongly associated with viral load and lung injury in patients with severe COVID-19. Ang II contributes to endothelial dysfunction, the development of arteriosclerosis, and microvascular thrombosis. Ang II infusion leads to abnormal inflammatory and thrombotic reactions in the microcirculation. In hypertensive rats, Ang II accelerates arterial thrombosis of the carotid artery via the AT1 receptor.