Human apoptosis protease activating factor-1,Apaf-1 ELISA Kit

Code CSB-E08996h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O14727
Lead Time 3-5 working days
Abbreviation APAF1
Protein Biological Process 1 Apoptosis/Autophagy
Target Name apoptotic peptidase activating factor 1
Alias APAF-1, CED4, DKFZp781B1145, apoptotic protease activating factor 1
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
HGNC 17581
RGD 1564562
MGI 1926788
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Apaf-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %88-98
1:2Average %95
Range %91-99
1:4Average %85
Range %80-89
1:8Average %94
Range %88-98
Recovery
The recovery of human Apaf-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-102
EDTA plasma (n=4)8682-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.590 2.396 2.493 2.337
15001.664 1.565 1.615 1.459
7501.064 1.008 1.036 0.880
3750.652 0.673 0.663 0.507
187.50.427 0.414 0.421 0.265
940.287 0.295 0.291 0.135
470.222 0.217 0.220 0.064
00.157 0.155 0.156
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Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Subcellular Location Cytoplasm
Tissue Specificity Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Database Links

HGNC: 576

OMIM: 602233

KEGG: hsa:317

STRING: 9606.ENSP00000448165

UniGene: Hs.552567

Pathway p53 signaling pathway
Apoptosis

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